小容量高渗氯化钠羟乙基淀粉40注射液对小鼠局灶性脑缺血再灌注损伤的影响

作    者:张蓬勃, 王妮, 高明, 李玲, 李卫松, 雷珊, 李旭, 郑娟, 刘鹏斌
单    位:西安交通大学医学院第二附属医院
基金项目:国家自然科学基金资助项目(30772081);
摘    要:
【摘要】 目的 研究高渗氯化钠羟乙基淀粉40注射液对小鼠局灶性脑缺血再灌注损伤的影响。 方法 30只昆明种小鼠,随机分成三组,每组10只:高渗氯化钠羟乙基淀粉40组(HSH组)、羟乙基淀粉130/0.4组(HES组)、对照组(Control组)。于缺血后即刻经尾静脉注射不同药物:HSH组给予4ml/kg高渗氯化钠羟乙基淀粉40注射液; HES组给予4ml/kg羟乙基淀粉130/0.4氯化钠注射液;Control组给予4ml/kg的生理盐水。缺血后12、 24、72h行神经行为学评分;24h 进行TTC染色,计算脑梗死面积百分率;72h甲苯胺蓝染色,检测半暗带内存活神经原元密度。 结果 三组小鼠的神经行为学评分、梗死面积百分率以及半暗带内存活神经原元密度组间比较均无统计学差异(p>0.05)。结论 高渗氯化钠羟乙基淀粉40注射液对小鼠局灶性脑缺血再灌注损伤无改善作用。
关键词:羟乙基淀粉130/0.4氯化钠注射液;高渗氯化钠羟乙基淀粉40注射液;脑缺血再灌注损伤;半暗带

Effect of small volume hypertonic sodium chloride hydroxyethyl starch 40 injection on focal cerebral ischemia-reperfusion injury in mice

Author's Name: 
Institution: 
Abstract:
【Abstract】 Objective To investigate the effect of the hypertonic sodium chloride hydroxyethyl starch 40 injection on focal cerebral ischemia-reperfusion injury in mice. Methods Thirty KM mice were divided into three groups(10 in each group) randomly: hypertonic hydroxyethyl starch 40 sodium chloride group (group HSH), hydroxyethyl starch 130/0.4 group (group HES), control group (group control). At onset of ischemic insult, different agents were injected via the tail vein instantly. Mice in group HSH received 4ml/kg of hypertonic hydroxyethyl starch 40 sodium chloride injection. Mice in group HES received 4ml/kg of hydroxyethyl starch 130/0.4 sodium chloride injection, Mice in group control received 4ml/kg of 0.9% saline injection. Neurological behavioral testing was performed at 12, 24 and 72 h after ischemia, respectively. TTC staining was performed at 24 h after ischemia, and the percentage of infarction area was calculated. Toluidine blue staining was performed at 72h after ischemia and surviving neurons in the penumbra were counted. Results There were no difference in neurological scoring, percentage of infarct areas and density of surviving neurons in the penumbra among three groups, respectively. Conclusion Hypertonic hydroxyethyl starch 40 sodium chloride injection has no effect on focal cerebral ischemia-reperfusion injury in mice.
Keywords: Hydroxyethyl starch 130/0.4 sodium chloride injection;hypertonic sodium chloride hydroxyethyl starch 40 injection;ischemia-reperfusion injury;penumbra
投稿时间:2010-06-28  


 
 
 
 
 
 
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