目的 研究骨骼肌内质网应激对大鼠开胸手术后胰岛素抵抗的影响。 方法 建立大鼠开胸手术模型,采用随机数字表法将24只SD大鼠分为4组(每组6只):对照组、4-苯基丁酸钠(4-phenyl butyric acid, 4-PBA)组、手术组、4-PBA+手术组。手术次日抽血检测血清游离脂肪酸,行高胰岛素-正常血糖钳夹实验,记录葡萄糖输注速率(glucose infusion rate, GIR)。取比目鱼肌检测内质网应激标志蛋白[重链结合蛋白(binding immunoglobulin protein, BiP)]。 结果 术后手术组大鼠血清游离脂肪酸、血糖、胰岛素、高胰岛素-正常血糖钳夹实验GIR及BiP的表达显著高于对照组、4-PBA组和4-PBA+手术组(P<0.05)。 结论 内质网应激参与了大鼠开胸手术术后的胰岛素抵抗,内质网应激抑制剂4-PBA可以缓解术后引起的胰岛素抵抗。
Objective To investigate the role of endoplasmic reticulum stress in skeletal muscle in surgery-induced insulin resistance in rats. Methods The rat models of open-chest surgery were created. Twenty-four rats were randomly divided into 4 groups (n=6): control group, 4-phenyl butyric acid (4-PBA) group, operation group and 4-PBA+operation group. Rats in each group were treated with hyperinsulinemic-euglycemic clamp test to assess insulin sensitivity 1 day after operation. Western blot assay was used for detecting the expression of binding immunoglobulin protein (BiP) in skeletal muscles. Results Compared with the control group, 4-PBA group and 4-PBA+operation group, the plasma free fatty acid level of the operation groups was elevated, while glucose infusion rate(GIR) was decreased, BiP was significantly increased in rat soleus samples (P<0.05). Conclusions Endoplasmic reticulum stress in skeletal muscles is involved in surgery-induced insulin resistance. 4-PBA, endoplasmic reticulum stress inhibitor, ameliorates surgery-induced insulin resistance in rats.
Cited