Ｏｂｊｅｃｔｉｖｅ　To explore the effects of parecoxib on the intestinal mucosa barrier in a mouse model of sepsis.　Methods　Cecal ligation and puncture procedure (CLP) was applied to induce sepsis. Seventy-two Wistar rats were randomly divided into 4 groups (n=18): Sham, Sham+10 mg/kg parecoxib (SP), CLP, CLP+10 mg/kg parecoxib (CP). Rats were intraperitoneally injected with 10 mg/kg parecoxib or same volume of saline 20 min after CLP or sham operation, twice a day. Twenty-four hours after operation, the activity of diamine oxidase (DAO), D-lactate, and intestinal tissue myeloperoxidase (MPO) was measured. The levels of intestinal tight junction protein including Claudin-1 and zonula occludens-1(ZO-1) were detected. The pathological alterations in the intestine were revealed with microscopy.　Results　Parecoxib treatment reduced the activity of MPO in the intestinal tissue, decreased the plasma level of DAO and D-lactate, and significantly attenuated the damage of the intestinal tissue(P<0.05 vs group CLP). Meanwhile, Parecoxib upregulated the expression of ZO-1 and Claudin-1 protein which may be associated with the homeostasis of the intestinal mucosa barrier(P<0.05 vs group CLP).　Conclusions　Parecoxib treatment improveds the intestine injury, and reduced the permeability of the intestinal mucosa barrier in sepsis.