Abstract: Objective To investigate the relationship between GABAA receptor and the hypnotic effects of pentobarbital sodium, sodium hydroxybutyrate, etomidate, propofol and ketamine. Methods After having established the mice model of hypnosis by intraperitoneally injected appropriate doses of pentobarbital sodium, sodium hydroxybutyrate, etomidate, propofol or ketamine, we intracerebroventricularly or intravenously injected the mice with different doses of bicuculline(Bic)or securinine (Se); and then observed effects on the sleeping time by using awaken test. Results Bic and Se significantly and dose-dependently decreased the sleeping time of mice treated with pentobarbital sodium, etomidate, propofol or ketamine(P<0.05, P<0.01), but had no distinctive effect on the sleeping time of mice treated with sodium hydroxybutyrate(P>0.05). Conclusion GABAA receptor may be important target for the hypnotic effect of pentobarbital sodium, etomidate, propofol and ketamine, but not be the targets for the hypnotic effects of sodium hydroxybutyrate.
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