Objective To study the effect of intrathecal injecting (i.t.) astrocytes inhibitor fluorocitrate (FC) and /or microglia inhibitor minocycline (MI) on pain and tumour in a murine model of bone cancer pain. Methods C3H/He mice with intrathecal catheter insertion and checkouted by lidocaine experiment randomly was divided into six groups (n=20) which included normal group, sham group, cancer pian group (received tumour cells inserted into the left calcaneus bone marrow cavity )+ Artificial CSF group, FC group, MI group, FC + MI group. One day after administration, continued for 21 days, and before and on the 3rd,5th,7th,10th,14th,21th day after operation, the mice were tested by mechanical behavior of high-sensitivity, determination Lengtong-mouse behavior, weighing the body weight and observed the bone damage of bone tumor by biopsy HE staining. Results Compared with normal group, on the 3rd day after operation, the cancer pain group had obvious statistical significance. And compared with ACSF group, on the 5th day after operation, the group intrathecal FC+MI, the pain threshold was significantly increased (P<0.01), the group intrathecal MI the pain threshold was also significantly increased (P<0.05). On the 14th day after operation, the group intrathecal FC, the pain threshold was significantly increased (P<0.05). Compared with normal group, the cancer pain group body weight decreased significantly (P<0.05); compared with the with the ACSF group, MI group, FC group and MI + FC group, the body weight of mice was reduced less, there was statistical significance (P<0.05). At the same time point, compared with each other, there was no significance within the cancer pain groups, through the tumor size and bone mass of the extent of the damage. Conclusion During the test dose, while mice were intrathecal fluorinated citric acid and / or minocycline can increase the pain threshold, thereby inhibiting its hyperalgesia and pain of a high-sensitivity, also it can inhibit the reduction of body weigh, but it had no effect on tumor growth and the extent of damage.