Objective The present experiment investigated if intrathecal administration of Roscovitine could affect bone cancer pain in mice. Methods 24 C3H/HeJ mice were divided randomly into group S (sham+ vehicle), group C (tumor+ vehicle) and group R (tumor+ Roscovitine). Every group has eight mice. Osteosarcoma NCTC 2472 cells were implanted into the intramedullary space of the right femurs of mice to induce ongoing bone cancer related pain behaviors. The sham group was inoculated by α-MEM without any cells. The mice were observed the changes of pain behaviors in group R, C and S, such as paw withdrawal mechanical threshold (MWT) and paw withdrawal thermal latency (TWL), at 1 , 6 , 24，48，72 h after intrathecal administration of 20 μg Roscovitine or 5 μl DMSO. Results The levelof PMWT and PTWL among every group had no difference before operation. At day7 afterinoculation，PMWTofgroup tumor decreased to（1.08±0.24）g，and at day 10 PTWL shortened to（12.7±1.4）s，and then the pain behaviors of group tumor showed aggravation along with the development of cancer pain. There was significant difference in the pain behavior thresholds between group sham and group tumor（P<0.05）. The level of PMWT［（0.70±0.19）g］and PTWL［（14.16±1.07）s］increased compared with group C and base level at 6h after intrathecal administration of 20 μg Roscovitine，and kept increasing until reaching maximum level after 12h，then the value decreased gradually，lowering to the level of group C after 72 h. Conclusion Intrathecaladministration ofroscovitine can relief bone cancer pain in mouse models.