目的 探讨大鼠内毒素性急性肺损伤时应用丙泊酚后处理对肺组织p-JNK的影响。方法96只雄性SD大鼠按数字随机表随机分为4组，每组24只。生理盐水对照组（A组）；内毒素致伤组（LPS 5 mg/kg 尾静脉注射，B组）；丙泊酚低剂量治疗组（丙泊酚2 mg/kg诱导后，4 mg﹒kg-1﹒h-1 维持，C组）；丙泊酚高剂量治疗组（丙泊酚4 mg/kg诱导后，8 mg﹒kg-1﹒h-1 维持，D组）。每组大鼠均在诱导后1、2、3、4 h时经放血处死大鼠（采用放血法随机活杀6只大鼠并留取肺组织标本），用免疫蛋白印迹法(western-blot)和免疫组织化学法（IHC）检测肺组织JNK磷酸化的水平。结果B组在LPS注射后各时点肺组织JNK磷酸化水平均较A组显著升高（ P＜0.05或P＜0.01），尤以3 h时最为显著（P=0.002＜0.01），而在应用丙泊酚处理后，虽然大鼠的肺组织JNK磷酸化水平也较A组同时点相比有显著的升高（ P＜0.05和P＜0.01），但和B组相比却有显著的降低（P＜0.05或P＜0.01），在3h时降低最为明显（P=0.008＜0.01）。结论 丙泊酚可以显著抑制内毒素性急性肺损伤大鼠肺组织中p-JNK的表达。显。结论 丙泊酚可以显著抑制内毒素性急性肺损伤大鼠肺组织中p-JNK的表达。
Objective: To investigate the effects of JNK activation posttreated by propofol on lipopolysaccharide-induced acute lung injury. Methods: 96 male SD rats were randomly devided into 4 equal groups (n=24), namely the control group(0.9% sodium chloride group); LPS group (intravenous injection of LPS 5 mg/kg); low dose of propofol group (2 mg/kg induced and 4 mg﹒kg-1﹒h-1 maintained); large dose of propofol group (4 mg/kg induced and 8mg﹒kg-1﹒h-1 maintained). Six rats were killed at each time point after LPS intravenous administration(saline in group A)and the lungs were harved and the activation of Phospho-JNK (p-JNK) was recorded in the lung tissues by the method of western-blot and Immunocytoche-mical (IHC). Results: The activation of p-JNK in the lung tissues increasecd significantly after LPS administration（ P＜0.05orP＜0.01） and at the time point of 3 h after after LPS administration the increasing was most(P=0.002＜0.01) ,but declined significantly after treatment with propofol and the activation of p-JNK in group D declined more than those in group C (P＜0.05orP＜0.01） and at the time point of 3 h after after LPS administration the degression was most(P=0.008＜0.01). Conclusions: Treatment with propofol can significantly attenuate the p-JNK expression in LPS-induced acute lung injury in rats.
Cited
)
