Objective To investigate the effects of hyperoxygen solution (HO) injected in vein on biochemical indexes of rats with acute carbon monoxide poisoning. Methods 30 SD rats were randomly divided into 5 groups, 6 in each group. Normal group (N group), carbon monoxide poisoning group (C group) and different dose of HO treatment groups at 1h after CO poisoning, 20ml/kg (H20 group), 25ml / kg (H25 group) and 30ml/kg (H30 group). CO poisoning model were established by intraperitoneal injection CO with 120ml/kg. C group and the treatment group were such poisoning rats, while N group were given the same dose of air. At the end of treatments, all the rats were collected 0.5ml of arterial blood for blood gas measurement and 3 ml of venous blood for the determination of sensitive biochemical indexes in brain, heart, liver, and kidney. Results CO 120ml/kg with intraperitoneal injection can cause severe hypoxemia after 1.5 hours, PaO2 decreased from the 96.62 ± 2.4 mmHg to 43.04 ± 4.13mmHg. After CO poisoning for 1d, C group had significantly increase in Neuron-specific enolase (NSE), troponin (cTnT), creatine kinase (CK), creatine kinase MB (CK-MB), lactate dehydrogenase (LDH ), α-hydroxybutyrate dehydrogenase (α-HBDH), total bilirubin (TBIL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyl transferase (GGT), blood urea nitrogen (BUN ) and creatinine (Cr) (P <0.01). When intravenous infused with different doses of HOS, Dissolved oxygen in plasma was significantly increased, PaO2 increased from the 43.04 ± 4.13 mmHg to 81.65 ± 3.85mmHg.all the enzymes were significantly lower than these in C group, although still were significantly higher than these in N group(P<0.05 or P<0.01). Moreover, all the enzymes were significantly lowest in group H30 with a significantly dose dependent relationship. Conclusion Intravenous infusion with hyperoxygen solution can reduce the biochemical indexes changes caused by acute CO poisoning in rat, and prevent the importance organs from damage.