Objective The present study explored whether propofol protected human erythrocyte from oxidative damage induced by hydrogen peroxide(H),which was analysed by phosphatidylserine(PS) exposure at erythrocyte surface and forward scatter（FSC）of erythrocyte in vitro. Methods Human erythrocytes at 2% of hematocrit Isolated from healthy volunteers were randomly divided into 5 groups(n=3 each) which were group C(Ringer solution alone),group H(H2O2 alone),group P10+H(10µM of propofol and H),group P50+H(50µM of propofol and H) and group P100+H(100µM of propofol and H). H2O2 was used at a concentration of 200µM in each group. Both erythrocyte PS exposure and cell volume were estimated from annexin V-binding and FSC by flow cytometry. Data were analysed in One-Way ANOVA by SPSS 13.0. Results H2O2 significantly induced Both erythrocyte PS-exposing( p＜0.001) and FSC-decreasing( p＜0.05),which were effectively inhibited by propofol at the range of 50-100µM( p＜0.001). Conclusions Propofol inhibites human erythrocyte eryptosis induced by H2O2, which may be involved in it’s protection of erythrocytes from oxidative stress.