【Abstract】 To investigate the protective effects of penehyclidine hydrochloride in different dosages on intestinal mucosa injury in rabbits with traumatic shock. Methods: The animal model of traumatic shock was established by Lamson's method. Thirty rabbits was randomly divided into five groups: control group(Con group), traumatic shock group resuscitation with normal saline (NS group), traumatic shock group resuscitation with penehyclidine hydrochloride in low dosages(0.05mg/kg, PHCL group)，middle dosages(0.15mg/kg, PHCM group) and high dosages(0.45mg/kg, PHCH group). At pre-shock, tele-shock, four time points after resuscitation, mean arterial pressure(MAP) and heart rate(HR) were monitored, plasma diamine oxidase(DAO) activity and lactic acid concentration were measured by spectrophotometry. The pathological changes of tissue samples of the intestine were observed under microscope. Results: There were no significant differences of MAP at pre-shock between five groups. At tele-shock, the MAP of rabbits in NS group, PHCL group, PHCM group, PHCH group significantly decreased, but there were no significant differences of MAP between groups. At T3~T6 time points, the MAP of rabbits in NS group(68.8±3.0, 67.5±5.3, 71.0±3.7 and71.0±2.4mmHg) was significantly lower than at pre-shock (94.3±1.7mmHg), in Con group(82.8±4.1, 89.8±6.6, 82.4±3.3 and 94.4±6.2mmHg) and PHCM group (83.2±13.6, 82.2±9.0, 83.8±8.9 and 85.4±8.1mmHg). There were no significant differences in HR at pre-shock and tele-shock between five groups. At T3~T6 time points, the HR of rabbits in NS group(185±11, 181±7, 164±13 and 164±13 bpm) were significantly lower than at T1(276±13 bpm), also significantly (P<0.05 or P<0.01) lower than in Con group and PHCM group. The plasma DAO activity and D-lactate concentration in NS group, PHCL group, PHCM group, PHCH group were significantly increased at T2~T6 time points. Furthermore, at T3~T6 time points, the plasma DAO activity (0.393±0.020, 0.586±0.017, 0.844±0.036 and 0.568±0.016 u/ml) and D-lactate concentration (8.292±0.364, 7.539±0.098, 5.991±0.180 and 7.108±0.372 mmol/L) in NS group were significantly higher than in PHCL group and PHCH group, that in PHCL group and PHCH group were significantly higher than in PHCM group(0.111±0.016, 0.302±0.020, 0.501±0.014 and 0.183±0.018 u/ml, 5.664±0.546, 4.609±0.292, 3.310±0.363 and 4.720±0.205 mmol/L) (P<0.05 or P<0.01). The result of pathology showed that intestinal mucosal injury of rabbits in PHCM groups was significantly reduced compared with NS group. Conclusions: The resuscitation of PHC combinition with normal saline can stabilize hemodynamics in rabbits with traumatic shock. There was a significant protective effect on intestinal mucosal injury induced by traumatic shock, especially in PHCM. The plasma DAO activity and D-lactate concentration can be uesed to assess the extent of intestinal mucosa injury and barrier function.