Abstract: The effect of mitochondrial calcium uniporter activity has relations with mitochondrial energy metabolism during cerebral ischemia/reperfusion injury. Zhao Qin *,Wang Shi-lei,Wang Peng,Li Yu, Li Shu-hong, Ling Yong, Yao Ru-yong, Guo Yun-liang .*Department of Anesthesiology,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266555,China
Corresponding author: Wang Shi-lei,Email:wshlei@yahoo.com.cn
Abstract:0bjective To study the relationship between effect of mitochondrial calcium uniporter activity and mitochondrial energy metabolism during cerebral ischemia/reperfusion injury in rats.Methods 40 male rats were divided randomly into four groups:control group,ischemia/reperfusion group, ruthenium red group and spermine group. The MCAO rats were generated by thread embolism of middle cerebral artery as described previously. Ischemia was induced for 2 h followed by 24 h of reperfusion,mitochondrial complex activity,mitochondrial membrane potential (ΔΨm), intracellular ATP level and intracellular reactive oxygen species (ROS) generation were determined after reperfusion. Results Mitochondrial complex activity,mitochondrial membrane potential (ΔΨm)and intracellular ATP level were decreased in ischemia/reperfusion group, ruthenium red group and spermine group compared with those in control group(P<0.01),but intracellular reactive oxygen species (ROS) generation were increased in the three groups(P<0.01). Ruthenium red treatment could improve the parameters above: compared with those in I/R group, mitochondrial complex activity,mitochondrial membrane potential (ΔΨm)and intracellular ATP level were obviously enhanced(P<0.01), intracellular reactive oxygen species (ROS) generation were decreased (P<0.01)in ruthenium red group; while in the spermine group,the parameters about energy metabolism were relatively poor,as compared to those in ischemia/reperfusion group.Condusion The inhibition of the activity of mitochondrial calcium uniporter can obviously improve mitochondrial energy metabolism during cerebral ischemia/reperfusion injury.
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