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摘要：
目的 研究七氟醚对发育中大鼠海马神经元凋亡的影响以及凋亡与细胞内钙离子的关系。 方法 48只1月龄SD大鼠采用随机数字表法随机分为3组（每组16只）：空气对照组（Sa组），载气对照组（Sc组）和3%七氟醚吸入4 h组（S4组）。建立七氟醚吸入模型，吸入结束后12 h断头取海马组织，苏木精-伊红（hematoxylin?蛳eosin， HE）染色观察海马神经元形态学改变，用流式细胞仪检测凋亡，激光共聚焦显微镜测神经元细胞内游离钙离子浓度。 结果 七氟醚吸入组海马凋亡神经元数目（84.8±7.5）明显高于对照组［Sa组：（1.8±1.0）；Sc组：（2.2±1.0）］，神经元内游离钙离子浓度和钙离子荧光像素值［（297±21），（373±70）］均高于对照组［Sa组：（83±13），（113±57）；Sc组：（88±18），（120±50）］，差异有统计学意义（P<0.05）。 结论 七氟醚可诱导幼龄大鼠海马神经元凋亡，可能是通过升高细胞内钙离子浓度而触发凋亡。
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关键词： 七氟醚，发育中大脑，流式细胞仪，细胞内钙离子，激光扫描共聚焦显微镜
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Abstract: Objective To explore if sevoflurane could induce neuronal apoptosis and its relationship with intracellular calcium. Methods In this study， 48 fasted male Sprague-Dawley rats were randomly assigned into three groups. Group Sa， rats were administered with air. Group Sc， rats were administered with 100% oxygen. Group S4， rats were administered with 3% sevoflurane for 4，12 h after administration with air， oxygen or sevoflurane， all rats were killed and the hippocampus were collected for study. Cell morphology of hippocampal neuron was observed with hematoxylin-eosin（HE） staining. The percentage of apoptotic cells was measured by flow cytometry and intracellular calcium concentration was measured with laser scanning confocal microscope. Results The percentage of apoptotic neuron in hippocampus in group S4（84.8±7.5） was higher than that in group Sa（1.8±1.0） and group Sc（2.2±1.0）（P<0.05）. Intracellular free calcium concentration and fluorescence intensity was higher in group S4［（297±21），（373±70）］ than in group Sa［（83±13），（113±57）］ and group Sc［（88±18），（120±50）］ （P<0.05）. Conclusions Sevoflurane could induce hippocampal neuron apoptosis with a potential mechanism of increasing intracellular calcium.
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Key words: sevoflurane，the developing brain，flow cytometer，intracellular calcium，laser scanning confocal microscope,
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