Objective To evaluate the efficacy and adverse effect of sugammadex versus neostigmine on reversal of amino-steroidal muscle relaxants induced neuromuscular block. Methods The relevant randomized controlled trials(RCTs) were searched in PubMed、EBSCO、Springer、Ovid、Embase、Cochrane Library、CNKI and WanFang Data from the data of their establishment to June 2013. The quality of the included studies was evaluated after the data were extracted by two reviewers independently, and then the Meta-analysis was performed by using RevMan 5.2 software. Result Six RCTs involving 533 cases were included. The mean time to recovery of the TOF ratio to 0.7、0.8 and 0.9 was significantly faster with sugammadex compared with neostigmine, regardless of moderate and deep neuromuscular blockade. Compared with neostigmine group, the percentage of drug-related adverse effect in patients was decreased in sugammadex group. Conclusion Both in moderate and deep neuromuscular blockade, the efficacy of sugammadex on reversal of amino-steroidal muscle relaxants induced neuromuscular block was much stronger than neostigmine, and more security than neostigmine.