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Abstract: Ｏｂｊｅｃｔｉｖｅ To investigate the role and mechanism of protein kinase B（Akt） in opioid-induced hyperalgesia（OIH） with an incisional pain model. Methods Sixty C57BL/6 male mice were randomly divided into 5 groups（n=12）： incision pain+ dimethyl sulfoxide（DMSO） group（group Ⅰ）， incision pain+remifentanil+DMSO group（group R）， Akt inhibitor Ⅳ 0.08 μg/10 μl group（group A1）， Akt inhibitor Ⅳ 0.16 μg/10 μl group（group A2） and Akt inhibitor Ⅳ 0.32 μg/10 μl group（group A3）. DMSO was the solvent of Akt inhibitor Ⅳ. Incisional pain model was made in the right paw of all the mice. In group R and group A1， A2， A3， subcutaneous remifentanil（0.04 mg/kg） was infused for 30 min while the surgery was being performed and intrathecal 10 μl DMSO（10％） with corresponding concentration of the Akt inhibitor Ⅳ 10 μl was injected respectively . The paw mechanical withdrawal threshold（PMWT） and the paw withdrawal thermal latency（PWTL） was tested at 1 d（T0） before and 6 h（T1），1（T2），2（T3），3（T4），5（T5），7 d（T6） after surgery. Results Compared with group I and the baseline value， PMWT and PWTL were significantly decreased after surgery except 7 d in group R and all group A（P<0.05）. Compared with group R， PMWT［（5.03±0.62），（6.10±0.86），（5.92±0.88），（6.01±1.02） g. （4.07±0.79），（4.73±0.48），（4.77±0.59），（4.86±0.56） g. （5.05±0.75），（5.99±0.63），（5.99±0.71），（6.00±0.81） g］ and PWTL［（0.48±0.06），（0.60±0.08），（0.61±0.07），（0.58±0.04） s. （0.38±0.07），（0.50±0.08），（0.48±0.06），（0.45±0.08） s. （0.37±0.09），（0.52±0.09），（0.49±0.12），（0.58±0.21） s］ were significantly increased after surgery except 7 d in groups A1，A2，A3（P<0.05）. While there was no significan difference among group A1，A2 and A3（P>0.05）. Conclusions Intrathecal injection of different doses of Akt inhibitor IV effectively alleviates hyperalgesia induced by remifentanil in non-dose-dependent way.
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