目的 探讨甘氨酸转运体-1（glycine-transporter 1， Gly-T1）抑制剂肌氨酸（sarcosine， Sar）在大鼠切口痛模型中对瑞芬太尼痛觉敏化效应的影响。 方法 雄性Sprague-Dawley（SD）大鼠36只，采用随机数字表法将其随机分为：瑞芬太尼+鞘内生理盐水组（RN组）、瑞芬太尼+鞘内肌氨酸组（RS组）、生理盐水+鞘内生理盐水组（NN组）、生理盐水+鞘内肌氨酸组（NS组），每组9例，按分组分别在切口痛术前给予瑞芬太尼或生理盐水尾静脉泵注，术后第1天给予肌氨酸或生理盐水鞘内注射。实验中采用VonFrey法分别测定各组大鼠术前机械缩足反射阈值（paw withdrawal mechanical threshold， PWMT）及术后2、4、12 h，第1、3、5、7天的PWMT。 结果 与NN组比较，RN组的PWMT在术后2、4、12 h及术后第1天分别为（0.21±0.04）、（0.63±0.21）、（1.33±0.50）、（3.31±1.03） g，显著降低（P<0.01）。而RS组在上述时间点与RN组比较，PWMT分别为（2.11±1.17）、（2.89±1.05）、（4.31±1.57）、（7.11±1.76） g，明显增高（P<0.01），且与NN组比较差异无统计学意义（P>0.05）。 结论 瑞芬太尼具有致切口痛大鼠的痛觉敏化作用，该作用可被Gly-T1抑制剂——Sar所减轻，故Gly-T1可能参与了切口痛模型大鼠瑞芬太尼痛觉敏化的效应机制。
Ｏｂｊｅｃｔｉｖｅ To investigate the roles of glycine-transpoter 1（Gly-T1） inhibitor in the mechanism of remifentanil-induced hyperalgesia in rats incision pain model. Methods Thirty-six male Sprague-Dawley（SD） rats were randomly allocated into 4 groups（n=9）： RN group， RS group， NN group and NS group. All the rats were anesthetized by isoflurane during the model establishing. The rats in group RN and group RS received remifentanil via tail vein while those in group NN and group NS received saline before the incision surgery. One day after the surgery， the rats in group RS and group NS received a intrathecal injection of sarcosine while those in group RN and group NN received saline. Then the paw withdrawal mechanical threshold（PWMT） was detected from pre-operation to the 7th postoperative day. Results Compared to group NN， PWMTs were significantly decreased at 2， 4， 12 h and the 1th postoperative day（0.21±0.04），（0.63±0.21），（1.33±0.50），（3.31±1.03） g， respectively after incision in group RN（P<0.01）. Compare to group RN， the levels of PWMT（2.11±1.17），（2.89±1.05），（4.31±1.57），（7.11±1.76） g were significantly increased in group RS（P<0.01）. And there were no difference between group RS and group NS（P>0.05）. Conclusions On incision pain models in rats， remifentanil given systemically induces mechanical hyperalgesia， which can be ameliorated by Gly-T1 inhibitor sarcosine.
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