目的 观察骨癌痛大鼠脊髓水平磷酸化核转录因子-κB（phospho-nuclear factor-κB， p-NF-κB）的表达位置及表达变化。 方法 雌性未交配Sprague-Dawley（SD）大鼠48只，体重160 g~200 g，采用随机数字表法将其随机分为两组：假手术组（S组）、骨癌痛（bone cancer pain， BCP）组（BP组），每组24只。选用von Frey纤维丝检测各组大鼠造模前及造模后3、7、14、21 d机械缩足阈值（mechanical withdrawal threshold， MWT），评价疼痛行为学变化。采用免疫荧光法检测S组和BP组术前1 d及术后7、14、21 d大鼠脊髓L4~6节段背角Ⅰ、Ⅱ板层p?蛳NF?蛳κB阳性细胞数的变化。免疫荧光双标法检测脊髓L4~6节段背角Ⅰ、Ⅱ板层p-NF-κB与神经元标志物神经元特异核蛋白（neuronal nuclei， NeuN）、小胶质细胞标志物离子钙接头蛋白分子-1（ionized calcium binding adaptor molecule-1， Iba-1）、星形胶质细胞标志物胶质纤维酸性蛋白（glia fibrillary acidic protein， GFAP）共表达情况。 结果 各组肿瘤细胞接种前MWT比较差异无统计学意义（P>0.05）；与S组比较，BP组肿瘤细胞接种后第7天MWT［（3.57±1.29） g］、第14天MWT［（1.66±0.39） g］、第21天MWT［（1.40±0.16） g］降低（P<0.05）；S组各时间点MWT差异无统计学意义（P>0.05）。与S组比较，BP组大鼠术后7 d脊髓L4~6节段背角Ⅰ、Ⅱ板层p-NF-κB表达水平升高，随时间延长逐渐升高（P<0.05）。p-NF-κB几乎都与GFAP共表达，而很少与NeuN、Iba-1共表达。 结论 BCP大鼠脊髓背角水平星形胶质细胞内p-NF-κB参与介导BCP大鼠机械痛敏。
Ｏｂｊｅｃｔｉｖｅ To investigate the expression of spinal phospho-nuclear factor-κB（p-NF-κB） in a rat model of tibial bone cancer pain. Methods Forty-eight female Sprague-Dawley（SD） rats weighting 160 g-200 g were randomly divided into two groups： sham operation group （group S） and bone cancer pain（BCP） group（group BP）. Before operation and at 3，7，14，21 d after operation， changes of pain behavior of rats in each group were measured using von Frey filaments. The rats were sacrificed on one day before and 7，14，21 d after operation and L4-6 segments of the spinal cords were removed for measurement of the expression of p-NF-κB protein by fluorescent immunohistochemistry. Double immunostaining for p-NF-κB with cell-specific markers： neuronal nuclei（NeuN）， glia fibrillary acidic protein（GFAP） and ionized calcium binding adaptor molecule-1（Iba-1） was performed to identify the cell types that expressed p-NF-κB. Results Behavioral assays reveals that the mechanical withdrawal threshold（MWT） was significantly decreased at 7 d［（3.57±1.29） g］，14 d［（1.66±0.39） g］， 21 d［（1.40±0.16） g］ after operation（P<0.05） and decreased gradually with the progress of the disease in the group BP. Compared with that in group S， the expression of p-NF-κB was significantly increased at 7 d after operation（P<0.05） and increased gradually afterwards in the group BP. p-NF-κB was primarily coexpressed with GFAP， but little with NeuN and Iba-1 of group BP rats. Conclusions The expression of p-NF-κB in spinal astrocytes was involved in the development of tibial bone cancer pain.
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