目的 观察地佐辛在小鼠坐骨神经分支高选择结扎切断 (spared nerve injury, SNI)诱导的神经病理性疼痛与氯喹激发的急性瘙痒共病(co-morbidity of pain-itch, CPI)中的治疗效果。方法 成年雄性SPF级昆明小鼠，体重(22-26) g，随机分为6组(n=8)：A组，假手术－非瘙痒－生理盐水治疗对照组（Sham+NS+NS）；B组，神经病理性疼痛－非瘙痒－生理盐水治疗对照组（SNI+NS+NS）；C组，神经病理性疼痛－瘙痒－生理盐水治疗对照组（SNI+Itch+NS）；D组，假手术－瘙痒－生理盐水治疗对照组（Sham+Itch+NS）；E组，神经病理性疼痛－瘙痒－地佐辛治疗组（SNI+Itch+Dezocine）；F组，假手术－瘙痒－地佐辛治疗组（Sham+Itch+ Dezocine）。于SNI术前1d,术后1d, 3d, 5d（地佐辛/氯喹注药前、后)，采用电子VonFrey测定各时间点小鼠机械缩足阈值（pal withdrawal threshold, PWT）评估机械痛敏；采用视频记录并分析动物因氯喹激发的搔抓行为。结果 各组机械痛敏/镇痛效应PWT曲线下面积(AUC)比较：F-AUC>E-AUC>B-AUC；D-AUC>C-AUC (P<0.05)。搔抓潜伏期（scratching latency, SL）比较：A组（49±11）秒，B组（51±10）秒，C组（322±127），D组（333±94）秒，E组（571±67）秒，F组（524±77）秒；地佐辛治疗延长小鼠的搔抓潜伏期（P<0.05）。搔抓次数比较：C组（13.67±3.39），D组（25.78±5.21），E组（5.78±1.99），F组（6.33±1.80），C组搔抓次数< D组搔抓次数（P<0.05），E、F组搔抓次数< C、D组搔抓次数（P<0.05）。结论 对于小鼠CPI的治疗，地佐辛具有显著的镇痛与抗瘙痒作用。
Objective To observe the therapeutic Efficacy of dezocine on the treatment for the co-morbidity of neuropathic pain and itching, which induced by spared nerve injury (SNI) and evoked by chloroquine intradermal injection. Methods Adult male Kun-Ming mice (SPF grade, 22–26-g), were assigned randomly to six groups (n = 8): Group A (Sham+NS+NS), Group B (SNI+NS+NS), Group C (SNI+Itch+NS), Group D (Sham+Itch+NS), Group E (SNI+Itch+Dezocine), and Group F (Sham+Itch+ Dezocine). Mechanical allodynia was assessed by using electron-Von Frey. To test pain threshold, Paw withdrawal threshold (PWT, to assess mechanical allodynia) was measured by ElectroVonFrey (IITC Life Science, USA) on -1d (baseline), 1 d, 3 d, and 5 d (before and after dezocine and chloroquine intradermal injection). Scratching behaviors of animals were video-recorded to analysis chloroquine-evoked itching in each group. Results The area under curve (AUC) of PWT for mechanical allodynia or analgesic responses, Group A-AUC
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