Abstract: Objective To investigate the protective effects of penehyclidine hydrochloride(PHC) on intestinal mucosa injury in rabbits with traumatic shock. Methods The animal model of traumatic shock was established by Lamson's method. Twenty-four rabbits were randomly divided into control group(Con group), traumatic shock with normal saline group(NS group), traumatic shock treated with penehyclidine hydrochloride group(PHC group) and anisodamine group(ANI group). At pre-shock (T1), the end of shock(T2), 0(T3), 2 h(T4), 4 h(T5), 6 h(T6) after resuscitation, MAP and HR were monitored, intestinal tissue inducible nitric oxide synthase(iNOS) activity and nitric oxide(NO) concentration were measured by spectrophotometry. The pathological changes of tissue samples of the intestine were observed under microscope. Results At T2 time point, The level of MAP of rabbits in 4 experimental groups were prospectively decreased(≤45 mmHg)(1 mmHg=0.133 kPa). Compared with PHC group and ANI group At T3-T6 time points, MAP in NS group was signifigantly lower than that before shock(P<0.05). At T3-T6 time points, HR in NS group was significantly lower than that at T1, also siginificangtly lower than that in the Con group and the PHC group(P<0.05). Compared with control group[(0.70±0.24) U/mg, (0.70±0.32) μmol/g], intestinal tissue iNOS activity[(4.39±0.44), (1.59±0.49), (1.62±0.62) U/mg] and NO concentration[(5.81±0.27), (2.10±0.24), (2.15±0.30 ) μmol/g] in NS group, PHC group and ANI group were significantly increased(P<0.05). intestinal tissue iNOS activity and NO concentration were significantly higher in NS group than those in PHC and ANI group(P<0.05). Conclusions PHC and ANI can stabilize hemodynamics in rabbits with traumatic shock. There was a protective effect on intestinal mucosa injury induced by traumatic shock. Inhibiting the iNOS activity and decreasing the NO contents might be related to the mechanism of protective effect during intestinal mucosa injury.
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