【Abstract】Objective : To study the application of levobupivacaine combined dexmedetomidinein labor analgesia and its influence on mother and infant. Methods: In our hospital, 120 nulliparous women with term singleton fetus with cephalic presentation were randomly divided into two groups, group A with levobupivacaine combined dexmedetomidinein vs. and the control group B with levobupivacaine combined sufentanil respectively. Epidural anesthesia was used in the two groups, and epidural tube connected with electronic analgesia pump. After entering the first labor of pregnant women , we gave them epidural puncture, then two groups were given with 0.1% Levobupivacaine 5ml + 0.5 μg/ml Dexmedetomidine and 0.1% Levobupivacaine + 0.5μg/ml sufentanil 5 ml respectively. After that, it was connected with the electronic analgesia pump, set the background dose 8ml/h, PCA 5ml/time, locked in 15 min. We observed two groups of vital signs, VAS score, maternal Bromage score, Ramsay scale, labor, postpartum hemorrhage and neonatal Apgar score. Results: The blood pressure mm Hg(1 mm Hg=0.133 kPa) and heart rate of the two groups were decreased after 30min (Tb) than before anesthesia (Ta), were 10.9±0.62, 11.5±0.72 and 70±9.83, 76±8.23 respectively, then the difference between two groups is statistically significant.(P ＜0.05).The analgesic effect of the two groups, VAS score, at (T1), (T2) and (T3) point were different with that at point of (T0) (P ＜0.05), which were 1.3±0.7、3.0±0.3、3.0±0.5 and 1.7±0.6、3.5±0.7、3.2±0.8 respectively. Two groups of each point sedative effect, Ramsay index observation group was obviously lower than the control groupstatistically (P ＜0.05), which were 2.0±0.3、2.0±0.0、2.0±0.0和3.0±0.0、4.0±0.0、4.0±0.0. Two groups of Bromage score, lasting time, the pregnancy outcome, postpartum hemorrhage and neonatal Apgar score no (P＞0.05).Conclusion: The method of levopbupivacaine combined dexmedetomidine for labor analgesia was safe, effective and comfortable, had no adverse effect on maternal and newborn.