[Abstract] Objective To observe the effect of “minimally invasive” intubation technology used in parathyroidectomy resection in tracheal intubation anesthesia. Methods 100 cases ASAⅢ～Ⅳ,were collected for thyroid gland resection under general anesthesia. Based on random number table,Cases were divided into minimally invasive group (L group) and control (C group), 50 patients in each group. General anesthesia was inducted by using Midazolam, Etomidate, Sufentanil and CIS-atracurium. After 2.5 min, the L group were ecposured of glottis by using video laryngoscopic and by using disposable laryngeal tube to throat around and lidocaine spray 2% 3ml of glottis. After continue mask pressure 2 min for support oxygen, the ID 7.0 superslide analgesia endotracheal tube was inserted. After 4.5 min, in the C group, normal laryngoscope was used to inserted ID 7.5 endotracheal tube.We Maintained continuous pumping of remifentanil anesthesia, propofol, intermittent intravenous CIS-atracurium. Record SBP, DBP,HR, SPO2 of before anesthesia induction (T0),intubation (T1), intubation (T2), after intubation 1min (T3), after intubation 3min (T4), respectively. And before and after intubation 10min. We extracted vein blood for detecting the content of adrenocorticotropic hormone (ACTH) and cortisol (CORT) (for cortex alcohol has day secretion rhythm, participation test surgery are arranged in the morning). Results During the intubation period, the SBP, DBP, HR of C group were significantly increased than that of L group (p<0.05),and the content of adrenocorticotropic hormone (ACTH) and cortisol (CORT) were increased significantly than that of L group(p<0.05) , Conclusions Minimally invasive catheterization technique can effectively reduce parathyroidectomy cardiovascular responses to tracheal intubation, reduce the release of stress hormones in the blood, and increase the safety.