国际麻醉学与复苏杂志   2017, Issue (9): 0-0
    
电针对脑缺血大鼠脊髓背角细胞凋亡的影响
杨代和, 张世欣, 林菊珊, 孙一华, 黄文, 潘培梅1()
1.福建省第二人民医院
Effect of Electric Acupuncture on Apoptosis in Cerebral Ischemia Rats’ Spinal Dorsal Horn
 全文:
摘要:

目的:观察穴位电刺激对脑缺血再灌注大鼠脊髓背角细胞凋亡的影响。方法:采用Longa线栓法建立大鼠左侧大脑中动脉缺血(MCAO)再灌注模型,随机数字表法分为对照组和电针组。电针组予针刺患侧“曲池”、“足三里”穴位,对照组不予干预。两组于造模后1d、3d、7d及14d共4个时间点随机选取10只大鼠,运用TUNEL法检测脊髓背角细胞凋亡,免疫组化检测Caspase3蛋白表达。结果:电针组和对照组两组脊髓后角细胞凋亡以及Caspase-3蛋白表达阳性细胞率,3d、7d和14d都比1d高(P<0.05或P<0.01);电针组与对照比较,1d细胞凋亡率没差别,3d、7d和14d凋亡细胞及Caspase3蛋白表达阳性细胞率均小,差异具有统计学意义(P<0.05)。结论:穴位电刺激可以降低脊髓背角细胞凋亡。

关键词: 电针;脑缺血;脊髓后角;凋亡;Caspase3
Abstract:

Observe the effect of acupoint electricity stimulation on apoptosis in cerebral ischemia-reperfused rats’ spinal dorsal horn. Methods: Build rat left middle cerebral artery occlusion (MCAO) reperfusion model by Longa suture method, and random number table includes control group and electric acupuncture group. “Quchi” and “Zusanli” acupoints on the affected side are acupunctured in electric acupuncture group, while it is free of intervention in control group. 10 Rats are selected randomly from 2 groups at 4 time points including 1d, 3d, 7d and 14d after being modeled, and then apoptosis in spinal dorsal horn is detected by TUNEL method, and Caspase3 protein expression is detected by immunohistochemistry. Results: The apoptosis in spinal dorsal horn and the rate of positive cells in Caspase-3 protein expression in both Group EA and Group Con at 3d, 7d and 14d are higher than 1d (P <0.05 or P <0.01); after the comparison between Group EA and Group Con, there is no difference in apoptosis rate at 1d, but the apoptosis in cornu posterius medullae spinalis and the rate of positive cells in Caspase-3 protein expression are low at 3d, 7d and 14d, there has statistical significance in difference (P<0.05).Conclusions: Acupoint electricity stimulation can reduce apoptosis in spinal dorsal horn.

Key words: Electric acupuncture; Cerebral ischemia; Spinal dorsal horn; Apoptosis; Caspase3