Ｏｂｊｅｃｔｉｖｅ　To investigate the efficacy of CT-guided low-temperature plasma-mediated radiofrequency ablation combined with collagenase in the treatment of prolapse of lumbar intervertebral disc.　Methods　A total of fifty-eight patients with prolapse of lumbar intervertebral disc received low-temperature plasma-mediated radiofrequency ablation combined with collagenase were retrospectively analyzed. Changes in the degree of pain before and after operation were evaluated by VAS. The duration of postoperative recovery and pain relief were recorded. Risk factors for postoperative pain relief were analyzed.　Results　All patients underwent successful puncture, and no neurological injury, intervertebral disc infection, and no complications such as spinal cord and vascular organ injury. Postoperative follow-up for 6 months, the mean VAS was 3 points after 2 weeks. The mean VAS was 2.6 points at 1 month postoperatively, which was statistically different from the 2 weeks postoperatively (P<0.05). The stable state was achieved at 3 months after surgery, with an average of 1.3 points, compared with 2 weeks after surgery. There was a statistical difference (P<0.05). The mean VAS was 1.2 points at 6 months after operation, and there was no significant improvement compared with 3 months after operation. The difference was not statistically significant (P>0.05). MacNab evaluation was excellent. A total of 30 patients (51.7%) had a good evaluation of 15 patients (25.8%), and 8 patients (15.5%) had a good evaluation. The patients with poor results were 5 patients (7%). The excellent rate reached 89.7%.　Conclusions　Low-temperature plasma-mediated radiofrequency ablation combined with collagenase can effectively treat prolapse of lumbar intervertebral disc. These two techniques are both characterized by safety and minimal trauma. Thus, this method is recommended for combined minimally invasive interventional therapy in addition to conservative therapy and open surgery.