Objective　To evaluate the double-point labeling under ultrasonography for right internal jugular vein puncture cannulation in infants.　Methods　One hundred and twenty infants scheduled for surgery were randomly divided into 3 groups(n=40): traditional anatomical location group (group M), patients′ right internal jugular vein puncture，were cannulated by using the traditional method of palpation of carotid pulsation and identification of other anatomic landmarks. Real-time ultrasound guidance group (group C), a two-dimensional ultrasound scanner image made for locating the puncture site of vessels was used with an operative probe of PHILIPS CX30 system, L12-4 probe unit, real-time boot operation. Ultrasound pre-labeling followed by operation group (group L), an ultrasonic pre-locating two points, which made for locating the puncture site of vessels was used with an operative probe of PHILIPS CX30 system, L12-4 probe unit. The number of attempts, success rate and incidence of complications were compared among three groups.　Results　There was no significant difference in one-time success rate between group C and group L, but the rates of both groups were higher than the rate of group M (P＜0.05). There was no significant difference in puncture times between group C and group L, but the times of both groups were lower than the time of group M (P＜0.05). Also, there was no significant difference in operation time between group C and group L, but the times in both groups were less than the time of group M (P＜0.05). The main complications were puncture hematoma and arterial injury, the incidence of hematoma and arterial injury in group M were significantly higher than that in group C and L (P＜0.05).　Conclusions　Two points pre-localization under ultrasonography for the right internal jugular vein puncture is superior to the anatomic landmark technique in terms of successful rate and complications.