Ｏｂｊｅｃｔｉｖｅ　To verify the diagnostic efficacy of the sequential organ failure assessment (SOFA) score in Sepsis 3.0 for urosepsis.　Methods　A total of 540 patients undergoing ureteroscopic procedures of the upper urinary tract in our hospital were enrolled in the study. They were divided into two groups as sepsis group and non-sepsis group based on whether patients developed to sepsis or not. The data such as gender, age, mean arterial pressure (MAP), oxygenation index, Glasgow Coma Score, total bilirubin, platelet, serum creatinine were recorded.　Results　Sepsis occurred in 17 patients, and the incidence was 3.15%. The monofactor analysis shows that the difference between the two groups in postoperative SOFA score, ΔSOFA (the difference between postoperative SOFA score and the preoperative SOFA score) were statistically significant (P<0.01). ΔSOFA has higher positive predictive value and lower positive likelihood ratio. Conclusions　The ΔSOFA has clinically significance in the diagnosis of urosepsis.