【Abstract】Objective To compare the efficacy and safety of glycopyrrolate versus atropine on bradycardia induced by neostigmine. Methods Patients scheduled for elective non-cardiac surgery were randomly assigned to experimental group and control group. Patients in experimental group were administered with glycopyrrolate at a dose of 0.008mg/kg and neostigmine 0.004mg/kg in 1 minute, whereas patients in control group received atropine at a dose of 0.016mg/kg and neostigmine 0.004mg/kg in 1 minute to reverse the reversal of neuromuscular block at the end of operation, respectively. Heart rate was recorded for 15 min after administration of the reversal mixture , the area under the time curve (AUC) of the difference between heart rate and baseline heart rate, heart rate per minute and the difference between heart rate and baseline heart rate were compared; The dose of atropine for rescue therapy after administration was also recorded; And the adverse events within 24 hours after operation were followed up. Results The AUC of the difference between heart rate and baseline heart rate was smaller in experimental group than that in control group(p<0.05); the difference between heart rate and baseline heart rate was lower in experimental group than that in control group; the time of heart rate around to the baseline was longer in experimental group than that in control group. There was no significant difference in rescue therapy and adverse reactions between the two groups. Conclution Glycopyrrolate and atropine were compared to prevent bradycardia when given in a mixture with neostigmine for the reversal of non-depolarizing neuromuscular block, glycopyrrolate is superior to atropine in maintaining heart rate stability.