【Abstract】 Objective To explore the teaching practice and effect of a training course of point‑of‑care ultrasound of the upper airway in clinical anesthesiologists, so as to provide an effective training program for clinical popularization of this technique. Methods Anesthesiologists without clinical experience in point‑of‑car airway ultrasound were selected as the training subjects. The point‑of‑care airway ultrasound training course was designed according to the teaching objectives, and carried out through theoretical and practical training. After training, the trainees were evaluated for their ability to recognize ultrasonic images after endotracheal intubation and practical ability to perform point‑of‑care ultrasound in the airway. Results A total of 52 trainees participated in the training. After training, the trainees showed a sensitivity of 82.7% [95% confidence interval (CI) 72.1%, 93.3%], a specificity of 90.4% (95%CI 82.1%, 98.7%), and an accuracy of 86.5% (95%CI 79.9%, 93.2%) in identifying static ultrasound images after tracheal intubation. The trainees showed a sensitivity of 92.3% (95%CI 84.8%, 99.8%), a specificity of 92.3% (95%CI 84.8%, 99.8%), and an accuracy of 92.3% (95%CI 84.8%, 99.8%) in identifying dynamic ultrasound images after tracheal intubation. The point‑of‑care airway ultrasound was 100% qualified. Furthermore, 100% of the trainees were satisfied with the training and 98.08% were confident to apply point‑of‑care airway ultrasound in determining the position of the tracheal tube. Conclusions The point‑of‑care airway ultrasound can assist anesthesiologists to quickly and accurately master the skills of airway ultrasound for determining the position of endotracheal intubation.