Objective To describe the use of the GlideScope for patients with cervical spine immobilization. Methods 84 selective operation patients（ASAI-II）with cervical spine injury or severe cervical spondylosis were assigned to intubation by GlideScope videolaryngoscopy via mouth cavity after intravenous introduced general anesthesia. Prior to intubation all patients were given a Cormack grade by a senior anaesthetist using a Macintosh laryngoscopy. The patient was then intubated using GlideScope videolaryngoscopy. The procedure time of intubation, number of intubating times and the side-effects were recorded. HR, SBP, DBP, MAP were measured before（T1）and after（T2）anesthesia induction, at the beginning（T3）, 1 minute（T4）and 3 minutes（T5）after tracheal intubation. Results In the GS group, Cormack grade was improved in the majority （71/84）of patients（P<0.05）. The success rate was 97.6%. The procedure time of intubation was 12 s-135 s，and the average time of intuation was （28.7±9.2）s. Intubation succeed one time in 78 patients, twice in 4 patients, and two other patients were turned to fibreoptic intubation. During the intubation, the HR, SBP, DBP were stable. No injury of respiratory tract was observed. Conclusion GlideScope videolaryngoscopy introducing intubation seems a useful device in patients with cervical spine immobilization for its high successful rate, rapid intubation and excellent reliability.