国际麻醉学与复苏杂志   2010, Issue (1): 13-15
    
GABA 受体在静脉全麻药催眠作用的作用
许鹏程 程伟 马丽丽 戴体俊1()
1.徐州医学院江苏省麻醉学重点实验室
Effcets of bicuculline or securinine on the hypnotic effects of intravenous anesthetics
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摘要：

目的 探讨γ- 氨基丁酸A（GABAA）受体与静脉全麻药戊巴比妥钠、羟丁酸钠、依托咪酯、丙泊酚和氯胺酮催眠作用的关系。方法 建立小鼠腹腔注射静脉全麻药催眠模型，在催醒实验中分别观察侧脑室注射不同剂量的GABAA受体阻断药荷包牡丹碱（Bic）和静脉注射不同剂量的GABAA受体阻断药一叶秋碱（Se）对小鼠睡眠时间（sleeping time，ST）的影响。结果 Bic和Se 能够剂量依赖性地缩短戊巴比妥钠、依托咪酯、丙泊酚和氯胺酮催眠小鼠的ST（P<0.05 或P<0.01），但不能缩短羟丁酸钠催眠小鼠的ST。结论 GABAA受体是戊巴比妥钠、依托咪酯、丙泊酚和氯胺酮催眠作用的重要靶位，可能不是羟丁酸钠催眠作用的靶位。
关键词： GABAA受体；静脉全麻药；荷包牡丹碱；一叶秋碱；催眠；催醒
Abstract:

Objective To investigate the relationship between GABAA receptor and the hypnotic effects of pentobarbital sodium, sodium hydroxybutyrate, etomidate, propofol and ketamine. Methods After having established the mice model of hypnosis by intraperitoneally injected appropriate doses of pentobarbital sodium, sodium hydroxybutyrate, etomidate, propofol or ketamine, we intracerebroventricularly or intravenously injected the mice with different doses of bicuculline（Bic）or securinine （Se）; and then observed effects on the sleeping time by using awaken test. Results Bic and Se significantly and dose-dependently decreased the sleeping time of mice treated with pentobarbital sodium, etomidate, propofol or ketamine（P<0.05, P<0.01）, but had no distinctive effect on the sleeping time of mice treated with sodium hydroxybutyrate（P>0.05）. Conclusion GABAA receptor may be important target for the hypnotic effect of pentobarbital sodium, etomidate, propofol and ketamine, but not be the targets for the hypnotic effects of sodium hydroxybutyrate.
Key words: GABAA receptor；Intravenous anesthetics；Bicuculline；Securinine；Hypnosis；Analepsia