国际麻醉学与复苏杂志   2010, Issue (3): 226-229
    
丙泊酚对脂多糖诱导人单核细胞表达 Peroxiredoxin-2蛋白和超氧化物歧化酶蛋白的影响
孙艺娟 唐靖 古妙宁1()
1.510515 广州， 南方医科大学附属南方医院麻醉科（孙艺娟、 唐靖、 古妙宁）
The effects of propofol on Peroxiredoxin-2 and SOD1 expression from human mononuclear macrophage cell induced by LPS
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摘要：

目的 研究丙泊酚对脂多糖（lipopolysaccharide, LPS）诱导人单核细胞（THP-1）表达Peroxiredoxin-2及超氧化物歧化酶(SOD1)的影响。方法 将体外培养的THP1细胞按照完全随机方法分为4组：正常对照组（C组）：LPS刺激组（L组）：加入LPS 1 mg/L；丙泊酚处理组（P组）：给予丙泊酚15 mg/L和丙泊酚预处理合并LPS刺激组（P+L组）：给予丙泊酚15 mg/L，1 h后再给予LPS 1 mg/L。通过Western blot 法分别检测各组内Peroxiredoxin-2及SOD1含量的变化，用四唑盐快速比色法（MTT）观察各组THP1细胞的存活率。结果 在LPS刺激12 h后收集细胞，Westernblot 结果显示与正常对照组（C组）、丙泊酚处理组（P组）、丙泊酚预处理合并LPS刺激组（P+L）3组相比，LPS刺激组（L组）中Peroxiredoxin-2和SOD1的表达量明显降低，而L组与P+L组比较，P+L组中Peroxiredoxin-2 和SOD1的表达量则明显高于L组；MTT结果显示L组与(L+P)组相比较，L组细胞存活率明显低于(L+P)组细胞存活率（P<0.05）结论 丙泊酚可以逆转LPS对THP1细胞表达抗氧化蛋白Peroxiredoxin-2及SOD1的抑制作用，其抗氧化作用可能与增加Peroxiredoxin-2及SOD1的表达有关。
关键词： 丙泊酚； 脂多糖；人单核细胞； Peroxiredoxin-2； 超氧化物歧化酶
Abstract:

Objective To explore effect of propofol on Peroxiredoxin-2 and SOD1 expression from human mononuclear macrophage cell（THP-1）induced by lipopolysaccharide（LPS）. Methods THP-1 cultured in vitro and they were randomly assigned to four groups: control group, LPS group (L group): LPS 1 mg/L; propofol group (P group): propofol 15 mg/L, LPS and pretreatment with propofol group (L+P group): propofol 15 mg/L,after 1 hour, adding LPS 1 mg/L. Using western blot to detect the expression of Peroxiredoxin-2 and SOD1, and using MTT to observe the survival rate of cell. Results 12h after stimulated by LPS, results in Westernblot show that the expression of Peroxiredoxin-2 and SOD1 were both significantly decreased in L group compared to other three groups, however Peroxiredoxin-2 and SOD1 were both higher in L+P group than in L group. Results in MTT show that the survival rate of cell in L group was significantly lower than LPS and pretreatment with propofol group（P<0.05）. Conclusion Propofol can increase the expression of Peroxiredoxin-2 and SOD1, and it may have some anti-peroxidation effects.
Key words: Propofol; Lipopolysaccharide; Human mononuclear macrophage cell; Peroxiredoxin-2; SOD1