国际麻醉学与复苏杂志   2013, Issue (3): 1-1
    
氟比洛芬酯对骨癌大鼠肾功能、凝血功能及其血小板聚集的影响
胡正权, 吴刘萍, 马正良, 顾小萍1()
1.徐州医学院
Influences of flurbiprofen axetil on the kidney, platelet aggregation and coagulation function in rats with bone cancer
 全文:
摘要：

目的 连续7 d腹腔注射氟比洛芬酯注射液，研究其对骨癌痛大鼠肾脏、凝血功能、血小板聚集影响。方法 36只成熟雌性SD大鼠完全随机分为6组:肿瘤+生理盐水（C组）、肿瘤+氟比洛芬酯10 mg•kg-1•d-1组（CK10组)、肿瘤+氟比洛芬酯25 mg•kg-1•d-1组(CK25组)、肿瘤+氟比洛芬酯50 mg•kg-1•d-1组(CK50 组)、氟比洛芬酯50 mg•kg-1•d-1单纯组（K50组）和假手术组+生理盐水（sham组），每组6只。制作骨癌痛模型14 d后，每天分别腹腔注射相应剂量氟比洛芬酯或生理盐水2次，连续7 d后处死大鼠。腹主动脉采血，检测血尿素氮（blood urea nitrogen,BUN）、肌酐（creatinine,Cr）、Na＋、K＋、凝血酶原时间（protrombin time, PT）、活化部分凝血活酶时间（activated partial thromboplastin time, APTT）、纤维蛋白原（fibrinogen, Fib）、血小板聚集功能(platelet aggregation, PA)及其观察肾病理学变化。结果 腹腔给予氟比洛芬酯7 d后，分别与sham组和C组比较，CK50组（9.9±1.5） mmol/L、K50组（9.7±1.4）mmol/L血BUN水平明显增高（P<0.05），CK50组（137±8） mmol/L、K50组（138±8）mmol/L Na＋和CK50（3.9±0.3）mmol/L 、K50（3.9±0.4）mmol/L K＋显著降低（P<0.05），Cr、PT、APTT、Fib和PA值变化无统计学意义（P>0.05）；CK10、CK25组血BUN、Cr、PT、APTT、Fib、Na＋、K＋和PA值分别与sham组、C组比较，差异无统计学意义（P>0.05）。CK50、K50组病理学变化为肾小球缩小，肾小球毛细血管充盈不足；C组、sham组、CK10、CK25组肾组织结构清晰，未见异常变化。结论 腹腔重复注射不同剂量氟比洛芬酯对骨肿瘤大鼠凝血功能及其血小板聚集功能无明显影响，然而大剂量氟比洛芬酯(50 mg•kg-1•d-1)影响尿素氮、钠钾离子的代谢及肾小球毛细血血管充盈。
关键词： 氟比洛芬酯；肾功能；凝血功能；血小板聚集
Abstract:

Objective To investigate influences of flurbiprofen axetil on kidney, platelet aggregation and coagulation function. Methods Thirty-six healthy female Sprague Drawley (SD)rats were randomly divided into six groups(6 rats in each group):cancer+normal saline group(CN), cancer+flurbiprofen axetil 10 mg/kg group(CK10) , cancer+flurbiprofen axetil 25 mg/kg group(CK25), cancer+ flurbiprofen axetil 50 mg/kg group(CK50), flurbiprofen　axetil 50 mg/kg　group(K50), and sham+normal saline(sham). Flurbiprofen axetil or normal saline was administered intraperitoneally twice a day from day 14 to day 21 after injection of Walker256 tumour cells into one tibia. Rats were sacrificed to obtain blood from abdominal aorta on the 21th day. Then the levels of blood urea nitrogen (BUN), creatinine (Cr), sodion, Potassium ion, protrombin time（PT）, activated partial thromboplastin time （APTT）, fibrinogen (Fib), platelet aggregation (PA) were detected and pathological changes of kidney were observed. Results Respectively compared with the Sham and C groups, the levels of BUN in the CK50（9.9±1.5） mmol/L and K50（9.7±1.4）mmol/L groups increased obviously 7 days after intraperitoneal injection of flurbiprofen axetil ( P<0.05), and the levels of sodion in the CK50（137±8）mmol/L and K50（138±8）mmol/L groups and Potassium ion in the CK50（3.9±0.3）mmol/L and K50（3.9±0.4）mmol/L groups reduced significantly( P<0.05),and the levels of Cr,PT,APTT,Fib and PA were no statistical difference（P>0.05）. Respectively compared with the sham and C groups, the levels of Cr, BUN , sodion, Potassium ion ,Cr, PT, APTT, Fib and PA were no statistical difference obviously in the CK10 and CK25groups (P>0.05). The main pathological changes in the CK50 and K50groups were glomerular narrow and poor supplement with blood in capillaries, while in the Sham, C, CK10 and CK25 groups,there were no pathological changes observed. Conclusions Intraperitoneal injection of flurbiprofen axetil in a certain range of dose was not found to impair platelet aggregation and coagulation function in a model of bone cancer pain in the rat. However, in large dose,there were obviously influences on the metabolism of BUN, sodion and potassium ion as well asinfusion of capillaries of renal glomeruli in rats.
Key words: Flurbiprofen axetil;Kidney ;Platelet aggregation;Coagulation function