国际麻醉学与复苏杂志   2013, Issue (3): 4-4
    
线粒体钙单向转运体在脑缺血再灌注损伤中的作用与线粒体能量代谢有关
赵芹, 王士雷, 王鹏, 李瑜, 凌勇, 姚如永, 郭云良, 张鑫1()
1.青岛大学医学院附属医院
The effect of mitochondrial calcium uniporter activity has relations with mitochondrial energy metabolism during cerebral ischemia/reperfusion injury.
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摘要：

目的 探讨线粒体钙单向转运体在大鼠脑缺血/再灌注(I/R)损伤中的作用与能量代谢的关系。方法 40只雄性Wistar大鼠随机分为对照组、脑缺血再灌注组、脑缺血再灌注复合钌红组、脑缺血再灌注复合精胺组，线栓法建立大鼠大脑中动脉闭塞(MCAO)模型，缺血2小时，再灌注24小时后检测线粒体呼吸链酶复合体Ⅰ-Ⅳ活性、线粒体膜电位(ΔΨm)、组织中ATP含量和ROS的含量。结果 脑缺血再灌注组、脑缺血再灌注复合钌红组及脑缺血再灌注复合精胺组的粒体呼吸链酶复合体的活性、线粒体膜电位和组织中ATP的含量均低于对照组（P<0.01），而这三组的ROS含量高于对照组（P<0.01）。钌红能够改善上述指标:与脑缺血再灌注组相比，钌红组中线粒体呼吸链酶复合体的活性、线粒体膜电位和组织中ATP的含量明显升高（P<0.01及P<0.05），ROS的含量明显降低（P<0.01）;而精胺组中的能量代谢指标则均差于脑缺血再灌注组。结论 抑制线粒体钙单向转运体的活动可以明显改善脑缺血/再灌注损伤中线粒体的能量代谢。
关键词： 线粒体钙单向转运体 脑缺血/再灌注损伤 能量代谢
Abstract:

The effect of mitochondrial calcium uniporter activity has relations with mitochondrial energy metabolism during cerebral ischemia/reperfusion injury. Zhao Qin *,Wang Shi-lei，Wang Peng，Li Yu, Li Shu-hong, Ling Yong, Yao Ru-yong, Guo Yun-liang ．*Department of Anesthesiology，The Affiliated Hospital of Qingdao University Medical College，Qingdao 266555,China Corresponding author: Wang Shi-lei，Email:wshlei@yahoo.com.cn Abstract：0bjective To study the relationship between effect of mitochondrial calcium uniporter activity and mitochondrial energy metabolism during cerebral ischemia/reperfusion injury in rats．Methods 40 male rats were divided randomly into four groups：control group，ischemia／reperfusion group, ruthenium red group and spermine group. The MCAO rats were generated by thread embolism of middle cerebral artery as described previously. Ischemia was induced for 2 h followed by 24 h of reperfusion，mitochondrial complex activity，mitochondrial membrane potential (ΔΨm)， intracellular ATP level and intracellular reactive oxygen species (ROS) generation were determined after reperfusion. Results Mitochondrial complex activity，mitochondrial membrane potential (ΔΨm)and intracellular ATP level were decreased in ischemia／reperfusion group, ruthenium red group and spermine group compared with those in control group（P<0.01）,but intracellular reactive oxygen species (ROS) generation were increased in the three groups（P<0.01）. Ruthenium red treatment could improve the parameters above: compared with those in I／R group, mitochondrial complex activity，mitochondrial membrane potential (ΔΨm)and intracellular ATP level were obviously enhanced（P<0.01）, intracellular reactive oxygen species (ROS) generation were decreased (P<0.01）in ruthenium red group; while in the spermine group，the parameters about energy metabolism were relatively poor，as compared to those in ischemia／reperfusion group．Condusion The inhibition of the activity of mitochondrial calcium uniporter can obviously improve mitochondrial energy metabolism during cerebral ischemia/reperfusion injury.
Key words: ischemia/reperfusion injury mitochondrial calcium uniporter energy metabolism