目的 观察七氟烷对幼鼠不同脑区神经元细胞多ADP-核糖聚合酶-1（Poly(ADP-ribose) polymerase-1 , PARP-1）表达、远期学习记忆能力及空间探索能力的影响。 方法 出生后7d的wistar幼鼠105只随机分为模拟麻醉组（A组）、1%七氟烷麻醉2h组（B组）、1%七氟烷麻醉4h组（C组）、2%七氟烷麻醉2h组（D组）和2%七氟烷麻醉4h组（E组）。麻醉结束后即刻，每组随机选3只，左心室取血进行血气分析。麻醉结束6后，每组随机选6只幼鼠，分别取大脑皮层和海马组织，用western blot方法检测PARP-1蛋白表达量。其余实验动物，分别在幼鼠成长至5周，8周，14周时，进行悬崖逃避实验和旷场实验。 结果 各组实验动物无缺氧和明显的CO2蓄积。与A组相比，E组海马PARP-1蛋白表达量明显增加,其他实验组无明显升高（A组: 0.32±0.53, B组: 0.45±0.11, C组: 0.46±0.15,D组: 0.34±0.14, E组:0.80±0.34; P<0.05），各组皮层PARP-1蛋白表达量无明显差别（P>0.05）。各组大鼠三个阶段的悬崖逃避实验表现无显著差异（A组: 0.34±0.07, B组: 0.33±0.14, C组: 0.28±0.11,D组: 0.29±0.13 E组: 0.38±0.15; P>0.05）。5周时接受七氟烷麻醉的大鼠在旷场中平面活动及垂直活动均多于模拟麻醉幼鼠（平面活动时间/s：A组：431.4±31.5, B组;462.9±26.8,C组：447.6±31.0, D组：467.0±22.7, E组：473.3±24.7; P<0.05；垂直面进入时间/s：A组：112.3±37.1, B组; 169.1±46.0,C组：152.3±44.3, D组：149.6±26.2, E组：129.4±36.0; P<0.05）;8周和14周时，各组动物旷场表现差别无统计学意义（P>0.05）。5周、8周及14周时，各组动物悬崖逃避实验结果无差别（P>0.05）。 结论 2%七氟烷作用于发育期的幼鼠4h，可导致PARP-1表达增加，诱发海马神经元凋亡；使成长中幼鼠在陌生环境中活动增加，影响其空间探索认知能力。
Objective To investigate the effect of sevoflurane anesthesia on the expression of poly(ADP-ribose) polymerase-1(PARP-1) protein in various brain region of neonatal mice and it`s learning ability as adult. Methods One hundred and five neonatal wistar rats (7 days postnatal ,P7) were randomly divided into sham anesthesia group (group A),1% sevoflurane anesthesia for 2h group (group B ),1% sevoflurane anesthesia for 4h group (group C ),2% sevoflurane anesthesia for 2h group (group D) and 2% sevoflurane anesthesia for 4h group (group E). Arterial blood samples were obtained immediately at the end of anesthesia for blood analysis. The expression contents of PARP-1 protein in Cortex and hippocampus was measured by Western blot. Behavioral studies which include Hangklip-Escape Test and Open-Field Test were performed separately when the rats were 5-week-old, 8-week-old and 14-week-old. Results No significant hypoxia and hypercarbon dioxide had be seen in all the mice. The expression of PARP-1 protein in hippocampus area increased significantly in Group E than that in Group A(Group A: 0.32±0.53, Group B: 0.45±0.11, Group C: 0.46±0.15, Group D: 0.34±0.14, Group E:0.80±0.34; P<0.05). , while that in other brain area was no significant difference among all groups (Group A: 0.34±0.07, Group B: 0.33±0.14, Group C: 0.28±0.11, Group D: 0.29±0.13, Group E: 0.38±0.15; P>0.05). Rats exposed to sevoflurane had longer travel distance and time than those exposed to sham anesthesia in Open-field when they were 5 weeks old（Plane move time/s：Group A：431.4±31.5, Group B;462.9±26.8, Group C：447.6±31.0, Group D：467.0±22.7, Group E：473.3±24.7; P<0.05；Vertical entry time/s：Group A：112.3±37.1, Group B; 169.1±46.0, Group C：152.3±44.3, Group D：149.6±26.2, Group E：129.4±36.0; P<0.05），while the activity had no difference when they were 8 weeks and 14 weeks old. There was no significant difference in the Hangklip-escape test at any time. Conclusions Neonatal exposure to 2% sevoflurane for 4h could increase the expression of PARP-1, and induce cells apoptosis in rats' hippocampus, at the same time, sevoflurane damages their spatial cognition during their growths.
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