Objective: To observe the safety and efficacy of using tranexamic acid to prevent excessive blood loss after laparoscopic partial nephrectomy, as well as its influence on coagulation and renal function. Method: 40 patients who received laparoscopic partial nephrectomy using random number table, were randomly divided into two groups; one is the tranexamic acid group (Group T) and the other is control group (Group C), 20 cases each. Group T was given tranexamic acid 0.5g diluted in 100ml of normal saline; Group C was given the same volume of normal saline as Group T. Both groups administration were complete before anesthesia induction. The bleeding and transfusion volume，the content of fibrinogen during and after operation were recorded, as well as the results of routine blood test, renal and coagulation function test before and 24-hour-after operation. Results: The blood loss of Patients in group T about 70ml,group C about 200ml; postoperative blood loss in group T patients about 80ml, group C about 220ml (P<0.05). However, the postoperative coagulation function change between groups was not statistically significant (P>0.05). Conclusion: Tranexamic acid can significantly reduce blood loss and transfusion in laparoscopic partial nephrectomy, without significant side-effect to coagulation and renal function.