Objective To evaluate the effect of Discopo in orotracheal intubation in patients with ankylosing spondylitis by comparing intubation time and successful rate between Macintosh blade and Discopo. Methods 24 patients (ASAⅠ~Ⅱ) with ankylosing spondylitis scheduled for elective operation under general anesthesia requiring orotracheal intubation were investigated. After induction and complete muscle relaxation, repeated laryngoscopy was performed using Macintosh blade and Discopo in randomized sequence before patients were intubated with the other intubation laryngoscope. Information including patient’s characteristics (age, sex, BMI) and airway assessment features (Mallampati oropharyngeal grade, thyromental distance and mouth opening) were collected. Cormark-Lehane Grade (C/L Grade), best achievable glottic exposure time, orotracheal intubation time, and intubation successful rate were recorded. Results Discopo achieved better C/L Grade than Macintosh blade (P<0.05). Insufficient glottic exposure, defined as C/L Grade≥Ⅲ, was experienced in 13 patients (54.2%) with Macintosh blade while none with Discopo. Both best achievable glottic exposure time and inbutation time with Discopo [(12.9±5.9) s and (14.5±3.2) s] were less than those with Macintosh blade [(16.3±7.6) s and (45.2±43.2) s, P<0.05]. The successful rate of intubation with Discopo(100%) was higher than Macintosh blade (83.3%, P<0.05). Conclusion Compared with direct Macintosh blade, Discopo could achieve a better glottic exposure by less time and higher successful rate of orotracheal intubation in patients with ankylosing spondylitis.