【Abstract】 Objective The purpose of this experiment is to study the effects of different temperature of intraventricular irrigating fluid on hemodynamic in beagle dogs. Methods : Fifteen healthy beagles were randomly divided into control group (group T), room temperature group (group S) and low temperature group (group D). Standard anaesthetic monitoring included hemodynamic parameters , SpO2 , EtCO2 and rectal temperature after dogs were anesthetized with remifentanil and propofol. We recorded hemodynamic changes at 0min,10min, 20min, 30min, 40min, 50min after changing the fluid as T0,T10, T20, T30, T40, T50.Results There was no significant statistical difference in weight, height , rectal temperature and age. Compared with T0 in group T, HR and MAP decreased significantly at T20～T50 (P<0.05), MPAP, CVP, PAWP as well as CO decreased significantly at T40～T50 (P<0.05). Compared with T0 in group S, HR decreased significantly at T30～T50 (P<0.05), and MAP decreased significantly at T40～T50 (P<0.05). Compared with T0 in group D, HR and MAP decreased significantly at T50 (P<0.05), but increased significantly at T30 (P<0.05). HR and MAP at T30 were significantly different between group T and group D (P<0.05). After the experiment there were no neurologic impairment found. Conclusions The experimental results show that irrigation fluid at room temperature does not cause hemodynamic change. The low temperature (16-18℃) irrigation fluid would cause tachycardia and hypertension. Therefore, it is not recommended for use during ETV.