[摘 要] 目的：探讨丁酸钠（SB）对脂多糖（LPS）诱导的急性肺损伤（ALI）大鼠是否具有肺保护作用。方法：40只雄性SD大鼠随机分成四组（n=10）：对照组、SB组、LPS组和SB+LPS组。对照组静脉注射生理盐水2 ml/kg，SB组腹腔注射SB 500 mg/kg，LPS组静脉注射LPS 6 mg/kg，SB+LPS组于注射LPS后即刻和4小时分别腹腔注射SB 500 mg/kg。6h后观察肺组织病理学变化，测定肺组织湿干重比（W/D）、髓过氧化物酶（MPO）活性、丙二醛（MDA）、一氧化氮（NO）含量及血清TNF-α、IL-1β、IL-8、HMGB-1含量。 结果：与LPS组相比较，SB+LPS组肺组织损伤程度明显减轻，W/D下降（P<0.05）,组织MPO活性、MDA和NO含量显著降低（P<0.01），血清TNF-α、IL-1β、IL-8、HMGB-1含量降低（P<0.05）。结论：丁酸钠能够通过抑制中性粒细胞聚集和炎症因子的产生，抑制肺部脂质过氧化和硝基化效应，从而减轻肺水肿和细胞损伤，发挥肺保护作用。
ABSTRACT Objective：To investigate the protective effects of sodium butyrate on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Methods：Totally forty male SD rats were randomly divided into control group, SB group, LPS group and SB+LPS group . In control and SB group, the rats were given normal saline ( 2 ml/kg ) intravenously and SB ( 500mg/kg ) intraperitoneally. The LPS group were injected LPS ( 6 mg/kg ) intravenously, while the SB+LPS group separately received SB ( 500 mg/kg ) at 0 h and 4 h time points intraperitoneally after LPS was given at 0 h time point. The animals in each group were sacrificed after the models were completed successfully for six hours. Pulmonary histological changes were evaluated by hematoxylin-eosin stain and lung wet/dry weight ratios were observed. Concentrations of tumor necrosis factor (TNF-α) and interleukin (IL-1β and IL-8) and high mobility group box 1(HMGB-1)in plasma and concentrations of nitric oxide (NO) and myeloperoxidase (MPO) activity and Methyl Di Aide Hyde(MDA) in lung tissue homogenates were measured by enzyme-linked immunosorbent assay (ELISA ). Results: LPS induced marked lung histological injury and a significant increase in W/D ratio and MPO activity as well as MDA and NO levels n lung tissue homogenates（P<0.01）. TNF-α ,IL-1β, IL-8 and HMGB-1 showed a rising trend in LPS group（P<0.05）. However all of these changes were significantly attenuated by posttreatment with SB（P<0.05). Conclusions: Sodium butyrate exert protective effects by inhibiting neutrophil aggregation and cytokine production as well as lung lipid peroxidation and nitro effect.Sodium butyrate can also attenuated pulmonary edema. and cell damage.
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