目的 研究帕瑞昔布钠预先干预对经前期综合征(premenstrual syndrome，PMS)模型大鼠的作用。 方法 36只Wistar大鼠依阴道涂片法确定大鼠动情周期，分别将处于动情后期的大鼠采用完全随机分组方法分为：正常组（C组），模型组（M组），模型+帕瑞昔布钠组（A组）。采用慢性束缚应激法复制PMS模型，持续7 d。造模第5~7天，A组给予帕瑞昔布钠肌注0.72 mg/kg。造模成功后，于第8天处死大鼠。免疫组化检测大鼠子宫内膜组织环氧化酶-2（cycloxygenase-2, COX-2）、前列腺素E2 （prostaglandin E 2, PGE2）的表达，Western blot法检测大鼠大脑海马组织COX-2、PGE2的表达。 结果 ① M组和A组大鼠子宫内膜COX-2表达高于C组，差异有统计学意义（P<0.01）；与M组（0.46±0.11）比较， A组（0.31±0.12）子宫内膜COX-2表达显著降低32%（P<0.05）。 ② M组和A组子宫内膜PGE2表达较C组显著增加（P<0.01）；与M组（0.49±0.08）比较，A组（0.39±0.09）子宫内膜PGE2表达显著降低18.9%（P<0.05)。③ M组和A组大鼠海马组织COX-2表达高于C组，差异有统计学意义（P<0.01）；与M组（0.91±0.03）比较，A组（0.72±0.05）大鼠海马组织COX-2表达显著降低20.8%（P<0.05）。④ M组和A组大鼠海马组织PGE2表达较C组显著增加（P<0.01）；与M组（1.13±0.01）比较，A组（0.92±0.06）大鼠海马PGE2表达显著降低18%（P<0.05)。 结论 帕瑞昔布钠预先干预能降低PMS模型大鼠子宫内膜及大脑海马部位COX-2、PGE2的表达。
Objective To investigate the effects of pre-intervention parecoxib sodium on premenstrual syndrome（PMS） model rats. Methods 36 rats were randomly divided into 3 groups according to the results of Vaginal secretion smear, the normal group (group C), the model group (group M) , and the pre-intervention parecoxib sodium group (group A). PMS models were prepared by chronic restraint stress, which persist for 7 d. Group A was pre-administered parecoxib sodium0.72 mg/kg from the fifth day to the seventh day. All the rats were sacrificed at the eighth day. Then we applied immunohistochemical to detect the COX-2 and PGE2 expression on rat endometrium, and Western blot to detect the COX-2 and PGE2 expression on rat hippocampus. Results ① COX-2 expression on rat endometrium of group M and group A was significantly higher than group C（P<0.01）; COX-2 expression on the endometrium of group A （0.31±0.12） was 32% lower than group M（0.46±0.11）（P<0.05）. ② PGE2 expression on rat endometrium of group M and group A was significantly higher than group C（P<0.01）; PGE2 expression on endometrium of group A（0.39±0.09）was 18.9% lower than group M（0.49±0.08）（P<0.05). ③ COX-2 expression on hippocampus of group M and group A was significantly higher than group C（P<0.01）; COX-2 expression on hippocampus of group A（0.72±0.05）was 20.8% lower than group M（0.91±0.03）（P<0.05）. ④ PGE 2 expression on hippocampus of group M and group A was significantly higher than group C（P<0.01）, COX-2 expression on hippocampus of group A（0.92±0.06）was 18% lower than group M（1.13±0.01）（P<0.05). Conclusions Parecoxib sodium pre-intervention could decrease COX-2 and PGE2 expression on endometrium and hippocampus in premenstrual syndrome model rats.
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