国际麻醉学与复苏杂志   2014, Issue (3): 4-4
    
α-硫辛酸对急性重症胰腺炎大鼠脑保护作用的实验研究
于齐宏, 王君涛, 杜奕奇1()
1.第二军医大学附属长海医院消化内科
Protective effects of alpha-lipoic acid on brain injury induced by severe acute pancreatitis in rats
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摘要：

【摘要】 目的 观察α-硫辛酸对重症急性胰腺炎大鼠脑损伤是否具有保护作用并探讨相关机制。方法 采用经胰胆管注射5%牛磺胆酸钠诱发重症急性胰腺炎大鼠脑损伤模型，将36只SD大鼠随机分为假手术组（Sham组）、重症急性胰腺炎组(SAP组)和α-硫辛酸治疗组（α-LA组），造模12 h后观察各组大鼠脑组织水含量、肿瘤坏死因子-α(TNF-α)含量、丙二醛(MDA)含量、超氧化物歧化酶 (SOD)活性及核转录因子NF-κB水平的变化。结果 Sham组大鼠脑组织水含量、TNF-α含量、MDA含量、SOD活性分别为（74.17±1.45）%、375.30±79.47 pg/ml、0.91±0.07 nmol/ml、156.53±42.71 nU/ml；NF-κB蛋白表达水平为(3.2±1.1)%。SAP组大鼠脑组织水含量、TNF-α含量、MDA含量、SOD活性分别为（81.94±1.62）%、891.39±134.16 pg/ml、1.42±0.11 nmol/ml、97.04±33.62 nU/ml，NF-κB蛋白表达水平为（27.5±4.3)%；与Sham组相比，均有统计学差异(P<0.05)。与SAP组相比，α-硫辛酸干预可以显著减轻脑组织氧化损伤水平（α-LA组MDA含量与SOD活性分别为1.05±0.10 nmol/ml、118.15±37.93 nU/ml），减少细胞因子TNF-α含量（563.92±118.59 pg/ml）及下调核转录因子NF-κB表达水平(P<0.05)。结论 α-硫辛酸对重症急性胰腺炎诱发的大鼠脑损伤具有一定的保护作用。
关键词： α-硫辛酸；急性重症胰腺炎；脑保护
Abstract:

【Abstract】 Objective To investigate the effect of alpha-lipoic acid on brain injury induced by severe acute pancreatitis in rats. Methods Severe acute pancreatitis model was induced by retrograde injection of 5% sodium taurocholate into the pancreatobiliary duct.36 SD rats were randomly divided into sham operation group (sham group), severe acute pancreatitis (SAP group) and alpha-lipoic acid treatment group (alpha-LA group).Each rat brain tissue was taken 12 hour later to detect the content of tumor necrosis factor-alpha (TNF-α) and malondialdehyde (MDA), superoxide dismutase (SOD) activity and nuclear transcription factor NF- kappaB level changes. Results The content of brain water, TNF-α, MDA, SOD activity were (74.17±1.45)%, 375.30±79.47 pg/ml, 0.91±0.07 nmol/ml, 156.53±42.71 nU/ml respectively and the expression level of NF-κB protein was (3.2 ± 1.1)% in brain tissues of sham group.Compared with the sham group, these indicators significant changes in SAP group and the content of brain water, TNF-α, MDA, SOD activity and expression level of NF-κB protein were (81.94±1.62)%, 891.39±134.16 pg/ml, 1.42±0.11 nmol/ml, 97.04±33.62 nU/ml, (27.5±4.3)% respectively. Compared with SAP group, alpha-lipoic acid significantly reduced the level of oxidative damage (the content of MDA and SOD activity were 1.05±0.10 nmol/ml，118.15±37.93 nU/ml respectively), TNF-alpha content (563.92±118.59 pg/ml) and lowered nuclear transcription factor NF-κB expression in brain tissue (P<0.05). Conclusions The results suggest that alpha-lipoic acid can protect against brain injury induced by severe acute pancreatitis in rats.
Key words: Alpha-lipoic acid; Severe acute pancreatitis; Brain protection