[Abstract] Objective To evaluate the influence of intraoperative Sodium Potassium Magnesium Calcium and Glucose injection infusion on hepatic and renal function, blood electrolytes, blood glucose, lactate and coagulation function. Methods Eighty Patients with ASA grade I or II scheduled for elective general surgery or orthopedic surgery were randomly assigned to experimental group and control group using random number table. 500 ml of Sodium Potassium Magnesium Calcium and Glucose injection (in the experimental group) or Sodium Lactate Ringer injection (in the control group) was infused at the rate of 15 ml·kg-1h-1 before induction, followed by 10 ml·kg-1h-1 for 2 h, and then 8 ml·kg-1h-1 until the end of surgery. Hepatic and renal function, blood electrolytes and coagulation function were recorded before and after infusion. Blood glucose and lactate were measured at the time of before infusion, pre-induction, 1 h and 2 h after induction, and the end of infusion. Results There was no significant difference in the value of alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), total bilirubin (TBIL), blood urea nitrogen (BUN) or serum creatinine (Cr) before and after infusion between two groups. There was no significant difference in the concentration of Na+, K+, Cl- or Ca2+ before and after infusion between two groups, but the concentration of Mg2+ was higher after infusion in the experimental group (0.8±0.2 mmol/L vs. 0.7±0.3 mmol/L, P＜0.05). Blood glucose increased during surgery, and after surgery decreased to preoperative level. There was no significant difference in the prothrombin time (PT) and activated partial thromboplastin time (APTT) between two groups. Conclusions Sodium Potassium Magnesium Calcium and Glucose injection infusion did not have harmful effect on hepatic and renal function, blood electrolytes, blood glucose, lactate and coagulation function. Compared with sodium lactate Ringer injection, the concentration of Mg2+ was better maintained with this injection.