Abstract: Objective To investigate the effect of high dose of ambroxol on lipolysaccharide(LPS)-induced acute lung injury(ALI) in rats. Methods Twenty-four male BALB/C rats were randomly divided into 3 groups(n=8): group A served as control group and received normal saline intracheally. Group B and group C received intracheal instillation of LPS(3 ml/kg). At 6 h after LPS or saline instillation, ambroxol(100 mg/kg) were administered into the tail vein in group C, and normal saline were administered at the same volume in group A and group B. Another dose of amborxol or normal saline were administered 24 h later. At 48 h after LPS or saline instillation, lipoxin A4, tumor necrosis factor(TNF)-α, interleukin(IL)-10, total protein and polymorphonuclear cells count in bronchoalveolar lavage fluid, and wet/dry weight ratio and myeloperoxidase(MPO) in lung tissues were measured. The morphopathogical changes in lung of mice were observed by hematoxylin?蛳eosin(HE) staining. Results At 48 h after LPS or saline instillation, lipoxin A4[(332±19) ng/L] and IL-10[(36±6) ng/L] in bronchoalveolar lavage fluid in group C were higher than that of group B[(273±25),(9±3) ng/L]. Levels of TNF-α[(243±65) ng/L], total protein[(0.328±0.037) mol/L], and polymorphonuclear cells count[(39.99±14.41)×104/ml] in group C were lower than that of group B[(391±105) ng/L,(0.444±0.283) mol/L,(137.94±28.98)×104/ml]. Wet/dry weight ratio(3.65±0.18) and MPO[(6.4±0.4) U/g] in lung tissues in group C were lower than that of group B[(4.37±0.58),(137.9±29.0) U/g)]. Morphopathogical changes of lung tissues were attenuated significantly in group C more significantly than that of group B. Conclusions Large dose of ambroxol might have the therapeutic efficiacy on LPS-induced ALI in rats partly through promoting the resolution of inflammation.
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