Objective: To investigate the disciplinary orientation and construction of anesthesia nursing as well as clinical training of anesthetic nurse, so as to define the subject system system of anesthesia nursing which is consistent with Chinese national circumstances. Methods: The system of anesthesia nursing were established as eight subspecialtiesThe post of anesthesia nursing was established and divided into eight subspecialty[The system of anesthesia nursing were established as eight subspecialties], including clinical anesthesia care, post-anesthesia recovery care, pain related care, post-anesthesia visiting, cell salvage, drug administration, infection managementadministration , equipment and information administration. All subspecialties have team leader and predefined echelon. The responsibility of anesthesia nurses was defined. Twenty-eight working systems, thirty protocols, eighteen algorithms for special surgery, sixteen emergency plans and quality control system for anesthesia nursing were established, developed and improved during daily practice. Standardization protocol was established for training of anesthetic nurses. Various forms of continuing post-graduation medical education were developed. Results: sixty-seven anesthetic nurses completed the structured training and managed the skill of anesthesia care. The responsibility of anesthesia nursing staff was clarified and the enthusiasm of staff was aroused. The value of anesthesia nursing was thoroughly realized in clinical anesthesia. The satisfaction levels of patients, doctors and nurses were elevatedincreased. We firstly proposed the methods for constructing anesthesia nursing subspecialty system, also improved the anesthesia administration and anesthesia safety guarantee system, which will promote the development of both anesthesiology and anesthesia nursing and provide base for constructing anesthesia nursing subject. Conclusion: It is demonstrated that the methods for constructing anesthesia nursing proposed in the present study is consistent with the requirement of nursing development in Chinaour country. Furthermore, the development of anesthesia nursing is [yet]in its initial phase, and subjectsubject basement construction, specialized team,research platform and resources need further improvements.