Abstract: Objective To study the effect of dexmedetomidine on lipopolysaccharide(LPS)-induced apoptosis in human umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells were randomly divided into four group (n=20):normal control group (group C), dexmedetomidine group (group D), LPS group (group L), LPS plus dexmedetomidine group(group L+D). The cell viability and apoptosis was measured by MTT assay and flow cytometry respectively after 24 h of cell culture. The superoxide dismutase (SOD) activity and malonaldehyde(MDA) content was measured by xanthine oxidase method and thiobarbituric acid(TBA) test respectively. The expression of cleaved Poly-ADP Ribosy polymerase-1(PARP-1) protein was detected by Western blot. Results The cell survival rate in group L and group L+D were 0.95±0.08 and 1.08±0.10(P<0.05),which were decreased by 36% and 27% respectively when compared with group C. The apoptotic rate in group L and group L+D were(14.70±1.8)% and (8.80±1.1)%(P<0.05), which were increased by 2.6 times and 1.1 times respectively when compared with group C. SOD activity in group L and group L+D were(99±6) U/mg and(182±9) U/mg(P<0.05),which were decreased by 53% and 14% respectively when compared with group C. MDA content in group L and group L+D were (29.9±1.8) nmol/mg and (19.3±2.1) nmol/mg(P<0.05), which were increased by 1.6 times and 79% respectively when compared with group C. The expression of PARP-1 protein fragment in group L and group L+D were 1.152±0.095 and 0.564±0.045(P<0.05), which were increased by 4.8 times and 1.8 times respectively when compared with group C. No significant difference was found in group D when compared with group C(P>0.05). Conclusions Dexmedetomidine can effectively reduce LPS-induced apoptosis in human umbilical vein endothelial cells by inhibiting oxidative stress and down-regulating expression of PARP-1 protein fragment.
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