Ｏｂｊｅｃｔｉｖｅ　To accomplish the deep vein puncture operation quicker， safer and more convenient.　Methods　Selected 200 patients who need deep vein catheterization， due to operation， chemotherapy， parenteral nutrition， long-term infusion or others. The patients were divided into group A and group B according to the random number table method. Ordinary wire frame was used in group A patients， while bi-directional catheter guide wire frame in group B patients， and each group having 100 cases. The vein puncture methods from anterolateral axillary were used in both groups. Each patient was operated by the same doctor. The puncture failure or other puncture approach did not be selected. Then we observed punctures and counted the number of successful cases in one take， cases of the guide wire bending， cases of the other end of the guide wire reimplantation， the average operation time and cases of vascular injury.　Results　In group B， the number of successful cases in one take， cases of guide wire bending and cases of the other end of the guide wire reimplantation showed no remarkable difference from the number of group A（P>0.05）. The average operation time in group A was 7.5 min， 4.8 min in group B. Six patients suffered vascular injury in group A， while 2 patients in group B. When compared with the group A， the average operation time and vascular injury decreased significantly（P<0.05）.　Conclusions　The puncture with improved bi-directional catheter guide wire frame is a faster， safer， more convenient and reliable method， and fewer complications.