【Abstract】 Objective To evaluate the role of FGF-2/JNK pathway in rats with bone cancer pain(BCP). Methods Thirty two female SD rats were randomly divided into sham group(group S, n=8), BCP group(group B, n=8), BCP+PBS group(group BP, n=8), BCP+ FGF-2 neutralizing antibody group(group BA, n=8). The rat models of bone cancer pain were developed by intra-tibia inoculation of Walker 256 mammary gland cells. The rats of sham group were inoculated by saline without any cells. Before inoculation (T0) and on 3, 5, 7, 10, 12 and 14d(T1~6) after inoculation,paw withdrawal threshold to von Frey hair mechanical stimulation (PWMT) was measured.On 10, 11, 12d, the rats of group BP and group BA were intrathcal injected with drugs respectively. The rats were then sacriced and L4~5 segments of the spinal cord were removed for determination of p-JNK expression in spinal cord using western blot. Results Compared with group S,The significant decrease of PWMT was observed on T2~6 in group B and group BP(P<0.05), and on T2~4 in group BA(P<0.05). Compared with group B and group BP，the significant increase of PWMT was observed on T5~6 in group BA(P<0.05). Compared with group S, the expression of p-JNK was upregulated in group B and group BP(P<0.01). Compared with group B and group BP, the significant decrease of the expression of p-JNK was observed in group BA(P<0.01). Conclusion FGF-2/JNK pathway is involved in the mechanism of bone cancer pain of rats.