【摘要 】 目的 探讨红景天苷（salidroside,SDS）对机械通气导致的小鼠急性肺损伤是否具有保护作用。方法 28只7～9周的清洁级ICR雄性小鼠，按完全随机分组法随机分为4组(每组7只)：对照组(CON组)、机械通气组(VEN组) 、红景天苷组（SDS组）和红景天苷+机械通气组(SDS+VEN组)。VEN、SDS+VEN组机械通气4小时后或CON组和SDS组麻醉插管4小时后检测支气管肺泡灌洗液中（bronchoalveolar lavage fluid，BALF）蛋白浓度和细胞数量以及丙二醛（malondialdehyde，MDA）和超氧化物歧化酶（superoxide dismutase, SOD）的含量变化，测定肺组织湿/干重比（wet-to-dry weight ratio，W／D），观察肺组织病理改变，并用酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测肺组织中白细胞介素-1β(interleukin，IL1-β)、肿瘤坏死因子-α(tumor necrosis factor-a，TNF-α)的含量。结果 BALF中蛋白浓度和细胞数量：VEN组分别为（0.244 0.057）g/L和（2.02 0.33）个／ml，与CON组比较明显升高(P<0．01)；SDS +VEN组分别为（0.170 0.023）g／L和（1.56 0.28）个／ml，与VEN组比较显著下降(P<0.05)。VEN组肺组织W／D（5.11 0.12)与CON组（4.69 0.08）比较，差异有统计学意义(P<0.01)，SDS +VEN组肺组织W／D（4.77 0.05）与VEN组比较，差异有统计学意义(P<0.05)。SDS预处理能显著减轻通气诱导的肺组织病理损伤。机械通气4 h可诱导肺组织中IL-1β和TNF-α蛋白表达显著升高，SDS预处理可减轻机械通气诱导的IL-1β和TNF-α表达：SDS+VEN组（14.4 2.3）mg／g和（15.1 1.8）ng/g与VEN组（19.5 5.9）mg／g和（18.2 3.9）ng/g比较，差异有统计学意义（P<0．05）。与CON组相比，VEN组灌洗液中MDA含量明显增加，SOD活性降低，而SDS预处理则明显降低MDA含量，增加SOD活性： SDS+VEN组（1.89 0.32）mg／g和（41 4）ng/g与VEN组（2.57 0.60）mg／g和（32 5）ng/g比较，差异有统计学意义（P<0.05）。结论 机械通气前给予红景天苷可有效减少小鼠肺组织炎症细胞聚集，减轻肺水肿，机制可能与其抑制氧化应激水平相关。
【Abstract】Objective To investigate the effects and mechanism of salidroside on ventilator induced acute lung injury in mice. Methods tweenty-eight male ICR mice were randomly divided into 4 groups: group control (CON group，n=7)，Mechanical ventilation group(VEN group， n=7)， Salidroside group(SDS group，n=7)，Salidroside +Mechanical ventilation group (SDS+VEN group，n=7). After 4 hours ventilation，collecting bronchoalveolar lavage fluid（BALF） and detecting the contents of protein，inflammatory cell counts，malondialdehyde（MDA），superoxide dismutase（SOD） in lung lavage fluid，the wet-to-dry weight ratio （W／D）and morphopathogical changes of lung tissue were examined．Lung tissues were homogenated to detect the Interleukin(IL)-1β and tumor necrosis factor-α(TNF-α) level by enzyme-linked immunosorbent assay (ELISA)．Results The concentration of protein and cell counts in VEN group(0.244 0.057g／L and 2.02 0.33／ml) were much higher than CON group(P＜0.01)，and SDS+VEN group(0.170 0.023g／L) and (1.56 0.28／ml) were significantly reduced by comparing with VEN group(P＜0.05). Wet/dry weight ratio were much higher in VEN group(5.11 0.12) than CON(4.69 0.08，P＜0.01) and SDS+VEN group(4．77_+0．05，P＜0.05). Pre-treated with SDS could significantly attenuate ventilator induced lung tissue injury. Four hours mechanical ventilation markedly evelated the IL-1β and TNF-α level in lung tissue and SDS could decrease the expression of IL-1β and TNF-α，there were significant statistical difference between SDS+VEN group[(14.4 2.3)mg／g and（15.1 1.8）ng/g] and VEN group[(19.5 5.9)mg／g and（18.2 3.9）ng/g ] of IL-1β(P＜0.05) and TNF-α(P＜0.05). After 4 hours ventilation，the MDA content in lung lavage fluid was increased significantly，SOD activity w as decreased(P＜0.05) ,compared with the ventilation group(2.57 0.60mg／g and 32 5ng/g)，the MDA was decreased and SOD activity was increased in salidreside group(1.89 0.32mg／g and 41 4ng/g). Conclusion Our results indicate that pre-treated with salidroside could significantly attenuate mechanical ventilation induced lung edema and the perfusion of inflammatory cells in mice, which might partly through inhibiting the oxidative stress level.
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