Ｏｂｊｅｃｔｉｖｅ　To investigate the amount of meningioma stem cells in the salvaged blood and evaluate the safety of using autologous salvaged blood during the surgery of meningiomas.　Methods　The experiment was approved by the local ethical committee. 70 patients who accepted the operation of meningioma were selected. Autologous blood salvage was used throughout the surgery. The blood samples were blood around the meningioma(B1), blood in the salvaged container (B2) and blood after washing and centrifuging (B3). Make blood smear of each sample and observe the shape of cells using light microscopy. B1, B2 and B3 blood samples were detected by flow cytometry and direct immunofluorescence. Observe the expression of cluster of differentiation 133(CD133) and cluster of differentiation 166(CD166) of B1, B2 and B3 respectively.　Results　Meningioma stem cells were not found in B1, B2 and B3 blood samples. The results of flow cytometry showed that the expression rate of positive CD133 were 0.54%, 0.43%, 0.39% separately in B1, B2 and B3 blood, and the expression rate of positive CD166 were 3.46%, 3.58%, 2.75% respectively in B1, B2 and B3 blood. But the expression rate of both was zero in the three samples.　Conclusions　There were no expression of CD133 and CD166 in the salvaged blood. This fact largely proved that there were no meningioma stem cells in the salvaged blood.