Sepsis is a syndrome of life-threatening organ dysfunction caused by a dysregulated host response to infection. Neutrophils are essential players in the host defense against invading pathogens. This review provides an overview of neutrophils dysfunction and the underlying mechanism in the sepsis. Several investigations have shown that impairment of neutrophil migration to the site of infection occurs during sepsis, resulting in an inability of the host to eliminate the infection. On the other hand, the neutrophil accumulation contributes to tissue damage and organ dysfunction during sepsis. This review summarizes the role and the potential mechanism of neutrophils in the sepsis. Neutrophils play an important role in the development of sepsis. Thus, its specific mechanisms need to be further studied and explored to give full play to its proper medical value.