Ｏｂｊｅｃｔｉｖｅ　To investigate the protecting effect and mechanisms of electroacupuncturing Zusanli(ST36) on pulmonary microvascular endothelial cell(PMVEC) of septic rat.　Methods　One hundred and eight male Sprague-Dawley rats were randomly divided into 6 groups(n=18): sham group(group Sham), sepsis group (group LPS), acupuncture non-acupoint stimulation group(group non-EA), bilateral electroacupuncture Zusanli group(group EA), bilateral cervical vagotomy after sepsis group (group VGX), bilateral cervical vagotomy thus electroacupunctureing Zusanli group (group VGX/EA). Abdominal aortic blood samples were taken 0.8 ml at 3, 6, 9 h to measure plasma tumor necrosis factor-α(TNF-α) expression level. Lung tissues were collected to investigate the expression of Src-suppressed C kinase substrate (SSeCKS) mRNA and E-selection mRNA. The pulmonary microvascular endothelial cells were observed by transmission electron microscope at 6 h after LPS exposure.　Results　The expression levels of TNF-α in plasma, as well as SSeCKS mRNA and E-selection mRNA expression levels in lung tissue were obviously higher in other groups than the expression levels of these molecules in group Sham (P<0.05). Statistical difference in all above expression levels of these molecules between group non-EA and LPS at 3, 6 h and 9 h were not detected(P＞0．05). The expression levels of TNF-α, SSeCKS mRNA and E-selection mRNA in group EA were markedly lower than the expression levels in group LPS(P<0.05). Electroacupuncturing Zusanli after vagotomy, statiscal difference in all above expression levels of these molecules were not detected compared with group VGX(P＞0．05). Transmission electron microscopy observed EA at Zusanli distinctly reduced the dysfunction of microvascular endothelial cells.　Conclusions　Electroacupuncturing Zusanli can protect LPS induced PMVEC injury and exert protective effects via activation of cholinergic anti-inflammatory pathway (CAP).