Objective To observe the effect of electroacupuncture on postoperative pain and immune function in patients after laparoscopic hysteromyoma surgery. Methods A total of 120 patients who were diagnosed with uterine leiomyoma and underwent laparoscopic surgery from March 2018 to April 2020 were enrolled. According to the random number table method, they were divided into two groups (n=60): a control group and an observation group. The control group received shame electroacupuncture, while the observation group was treated with electroacupuncture. Both groups were compared for age, number of hysteromyoma, diameter of hysteromyoma, operation time and exhaust time, Visual Analogue Scale (VAS) score at post‑operative 1, 6 h and 12 h, the levels of CD3+ and CD4+ before operation and at post‑operative 1 and 3 days, and the incidence of adverse reactions (nausea, vomiting and abdominal distension). Results There was no significant difference in age, number of hysteromyoma, diameter of hysteromyoma between the two groups (P>0.05). The postoperative exhaust time of the observation group was shorter than that of the control group (P<0.05), and there was no statistical difference in operation time between the two groups (P>0.05). There was no statistical difference in VAS scores between the two groups (P>0.05), while the VAS scores in the observation group at post‑operative 6 h and 12 h were lower than those in the control group (P<0.05). Compared with those 1 h before operation, both groups showed increases in VAS scores 6 h after operation (P<0.05); there was no statistical difference between VAS scores at post‑operative 6 h and 12 h (P>0.05). There was no statistical difference in the level of CD3+ and CD4+ between the two groups before operation (P>0.05). The levels of CD3+ and CD4+ in the observation group were higher than those in the control group (P<0.05). Compared with those before operation, the levels of CD3+ and CD4+ in the observation group decreased one days after operation (P<0.05). Compared with those at post‑operative 1 day, the levels of CD3+ and CD4+ in the observation group increased at post‑operative 3 days (P<0.05). The incidence of postoperative adverse reactions in the observation group was lower than that in the control group (P<0.05). Conclusions Electroacupuncture can relieve the pain of patients after laparoscopic hysteromyoma surgery, enhance immune function, reduce postoperative adverse reactions, and promote postoperative rehabilitation.