Pancreatic cancer (PC) is a highly invasive malignant tumor of digestive tract. The early symptoms were insidious, and the late symptom mostly manifested as chronic visceral pain (VP). PC pain mechanism is complicated and involves many factors. Some studies have pointed out that mast cells (MCs) play an essential role in the occurrence and development of PC pain. At present, there is a few reports on the molecular mechanism of MCs involved in PC pain, mainly focusing on the role played by MCs degranulated substances. This article mainly reviews the research status of PC pain, and summarizes the role of MCs and their degranulated substances in PC pain, so as to deeply understand the regulatory role of MCs in PC pain, which provides a reference and new therapeutic targets for future basic research and clinical treatment.